黄酮类化合物非西汀、芹菜素、山奈酚、柚皮素、柚皮苷调节大鼠肝线粒体的呼吸活性和膜电位,抑制红细胞的氧化过程。

Q3 Biochemistry, Genetics and Molecular Biology Biomeditsinskaya khimiya Pub Date : 2023-11-01 DOI:10.18097/PBMC20236905281
A I Savko, T V Ilyich, A G Veiko, T A Kovalenia, E A Lapshina, I B Zavodnik
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引用次数: 0

摘要

黄酮类化合物是植物的次生代谢产物,是植物化学物质中含量最丰富的一类。本研究的目的是以肝线粒体和红细胞为研究对象,比较几种代表性的不同类别黄酮类化合物,非西汀、芹菜素、山奈酚、柚皮素、柚皮苷的抗氧化活性和调节特性。在2.5-25μM的浓度范围内,芹菜素、山奈酚、柚皮素和柚皮素剂量依赖性地防止700μM叔丁基氢过氧化物诱导的红细胞氧化损伤:脂质过氧化(LPO)产物的积累和谷胱甘肽GSH的氧化。对应于类黄酮浓度抑制红细胞膜LPO过程50%的IC50值,非西汀为3.9±0.8μM,山奈酚为6.5±1.6μM,芹菜素为8.1±2.1μM,柚皮素为37.8±4.4μM,而柚皮苷为64.7±8.6μM。类黄酮在细胞膜结构中的抗氧化作用明显高于细胞质。研究的所有黄酮类化合物(10-50μM)都能有效抑制分离的大鼠肝线粒体的呼吸活性,除山奈酚外,还能刺激Ca²⁺-诱导线粒体膜电位的耗散。环孢菌素A和钌红抑制类黄酮刺激的Ca²⁺-依赖性膜去极化,从而表明线粒体钙单转运蛋白和线粒体通透性转换开孔参与了类黄酮的作用。黄酮类化合物是一种具有抗氧化活性的氧化还原活性化合物,能够调节线粒体电位和呼吸活性,防止线粒体氧化应激。它们可以被认为是有效的药物或营养品。
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The flavonoids fisetin, apigenin, kaempferol, naringenin, naringin regulate respiratory activity and membrane potential of rat liver mitochondria and inhibit oxidative processes in erythrocytes.

Flavonoids, secondary plant metabolites, represent the most abundant heterogeneous group of phytochemicals. The aim of this study to compare antioxidant activity and regulatory properties of several representatives of different classes of flavonoids, fisetin, apigenin, kaempferol, naringenin, naringin, using liver mitochondria and erythrocytes as research objects. In the concentration range of 2.5-25 μM fisetin, apigenin, kaempferol, naringenin, and naringin dose-dependently prevented oxidative damage of erythrocytes induced by 700 μM tert-butyl hydroperoxide: accumulation of lipid peroxidation (LPO) products and oxidation of glutathione GSH. The IC50 values corresponding to the flavonoid concentration inhibiting the LPO process in erythrocyte membranes by 50%, were 3.9±0.8 μM in the case of fisetin, 6.5±1.6 μM in the case of kaempferol, 8.1±2.1 μM in the case of apigenin, 37.8±4.4 μM in the case of naringenin, and 64.7±8.6 μM in the case of naringin. The antioxidant effect of flavonoids was significantly higher in the membrane structures compared to the cytoplasm of cells. All flavonoids studied (10-50 μM) effectively inhibited the respiratory activity of isolated rat liver mitochondria and, with the exception of kaempferol, stimulated Ca²⁺-induced dissipation of the mitochondrial membrane potential. Cyclosporine A and ruthenium red inhibited flavonoid-stimulated Ca²⁺-dependent membrane depolarization, thus indicating that the mitochondrial calcium uniporter and the mitochondrial permeability transition pore opening were involved in the flavonoid effects. Flavonoids, as the redox-active compounds with antioxidant properties, are able to regulate mitochondrial potential and respiratory activity, and prevent mitochondrial oxidative stress. They can be considered as effective pharmacological agents or nutraceuticals.

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来源期刊
Biomeditsinskaya khimiya
Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.30
自引率
0.00%
发文量
49
期刊介绍: The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).
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