BioFire JI小组的多中心评估与改善急性骨关节感染的微生物学诊断有关。

IF 4.5 3区医学 Q1 MICROBIOLOGY International Journal of Medical Microbiology Pub Date : 2023-11-01 DOI:10.1016/j.ijmm.2023.151588

Llanos Salar-Vidal , Catarina Chaves , Ileana T. Dianzo-Delgado , Patricio Favier , Salvador Giner-Almaraz , María José Gómez-Gómez , Guillermo Martín-Gutiérrez , Isabel Pereira , Ana Rodríguez-Fernández , Patricia Ruiz-Garbajosa , Carlos Salas-Venero , Jaime Esteban

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引用次数: 0

摘要

骨关节感染（OI）的微生物学诊断对于成功的治疗至关重要。2021年7月至2022年10月进行了一项前瞻性多中心研究，包括262种疑似急性OI的滑膜液。进行BioFire关节感染小组多重PCR检测，并将结果与滑膜液标本的传统培养物进行比较。总共检测到136种微生物，14个样本的一种以上微生物呈阳性。在单菌感染（n=87）中，与培养的一致性为69%。在26个样品中，当培养结果为阴性时，多重PCR产生额外的阳性结果。它有助于检测挑剔的微生物，如金黄色葡萄球菌和淋球菌。这种多重PCR已被证明是一种有用的技术，可用于高度怀疑急性OI的患者，以快速和自动化的方式。

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Multicenter evaluation of BioFire JI panel related to improved microbiological diagnostics on acute osteoarticular infections

Microbiological diagnosis of osteoarticular infections (OI) is crucial for a successful treatment. A prospective multicenter study including 262 synovial fluids with suspicion of acute OI was performed between July 2021 and October of 2022. BioFire Joint Infection Panel multiplex-PCR test was performed and results were compared with conventional cultures of synovial fluid specimens. In total, 136 microorganisms were detected, and fourteen samples were positive for more than one microorganism. In monomicrobial infections (n = 87) agreement with culture was 69%. In 26 samples, the multiplex PCR yield an additional positive result when culture result was negative. It helped in the detection of fastidious microorganisms as K. kingae and N. gonorrhoeae. This multiplex PCR has proven to be a useful technique that can be used for patients with high suspicion of acute OI in a rapid and automated manner.

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来源期刊

International Journal of Medical Microbiology 医学-病毒学

CiteScore

9.70

自引率

0.00%

发文量

审稿时长

45 days

期刊介绍： Pathogen genome sequencing projects have provided a wealth of data that need to be set in context to pathogenicity and the outcome of infections. In addition, the interplay between a pathogen and its host cell has become increasingly important to understand and interfere with diseases caused by microbial pathogens. IJMM meets these needs by focussing on genome and proteome analyses, studies dealing with the molecular mechanisms of pathogenicity and the evolution of pathogenic agents, the interactions between pathogens and host cells ("cellular microbiology"), and molecular epidemiology. To help the reader keeping up with the rapidly evolving new findings in the field of medical microbiology, IJMM publishes original articles, case studies and topical, state-of-the-art mini-reviews in a well balanced fashion. All articles are strictly peer-reviewed. Important topics are reinforced by 2 special issues per year dedicated to a particular theme. Finally, at irregular intervals, current opinions on recent or future developments in medical microbiology are presented in an editorial section.