Melissa S McDaniel, Nicholas A Sumpter, Natalie R Lindgren, Caitlin E Billiot, W Edward Swords
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The genomes of these isolates were then compared with all publicly available <i>S. maltophilia</i> genome assemblies, and each isolate was then evaluated for colonization/persistence <i>in vivo</i>, both alone and in coinfection with <i>P. aeruginosa</i>. We found that while the overall genome size and GC content were fairly consistent between strains, there was considerable variability in both genome structure and gene content. Similarly, there was significant variability in <i>S. maltophilia</i> colonization and persistence in experimental mouse respiratory infections in the presence or absence of <i>P. aeruginosa</i>. Ultimately, this study gives us a greater understanding of the genomic diversity of clinical <i>S. maltophilia</i> isolates, and how this genomic diversity relates to both interactions with other pulmonary pathogens and to host disease progression. Identifying the molecular determinants of infection with <i>S. maltophilia</i> can facilitate development of novel antimicrobial strategies for a highly drug-resistant pathogen.</p>","PeriodicalId":49819,"journal":{"name":"Microbiology-Sgm","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10710838/pdf/","citationCount":"0","resultStr":"{\"title\":\"Comparative genomics of clinical <i>Stenotrophomonas maltophilia</i> isolates reveals genetic diversity which correlates with colonization and persistence <i>in vivo</i>.\",\"authors\":\"Melissa S McDaniel, Nicholas A Sumpter, Natalie R Lindgren, Caitlin E Billiot, W Edward Swords\",\"doi\":\"10.1099/mic.0.001408\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Stenotrophomonas maltophilia</i> is a Gram-negative emerging opportunistic pathogen often present in people with respiratory diseases such as cystic fibrosis (CF). People with CF (pwCF) experience lifelong polymicrobial infections of the respiratory mucosa. Our prior work showed that <i>Pseudomonas aeruginosa</i> promotes persistence of <i>S. maltophilia</i> in mouse respiratory infections. As is typical for environmental opportunistic pathogens, <i>S. maltophilia</i> has a large genome and a high degree of genetic diversity. In this study, we evaluated the genomic content of <i>S. maltophilia,</i> combining short and long read sequencing to construct nearly complete genomes of 10 clinical isolates. The genomes of these isolates were then compared with all publicly available <i>S. maltophilia</i> genome assemblies, and each isolate was then evaluated for colonization/persistence <i>in vivo</i>, both alone and in coinfection with <i>P. aeruginosa</i>. We found that while the overall genome size and GC content were fairly consistent between strains, there was considerable variability in both genome structure and gene content. Similarly, there was significant variability in <i>S. maltophilia</i> colonization and persistence in experimental mouse respiratory infections in the presence or absence of <i>P. aeruginosa</i>. Ultimately, this study gives us a greater understanding of the genomic diversity of clinical <i>S. maltophilia</i> isolates, and how this genomic diversity relates to both interactions with other pulmonary pathogens and to host disease progression. 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Comparative genomics of clinical Stenotrophomonas maltophilia isolates reveals genetic diversity which correlates with colonization and persistence in vivo.
Stenotrophomonas maltophilia is a Gram-negative emerging opportunistic pathogen often present in people with respiratory diseases such as cystic fibrosis (CF). People with CF (pwCF) experience lifelong polymicrobial infections of the respiratory mucosa. Our prior work showed that Pseudomonas aeruginosa promotes persistence of S. maltophilia in mouse respiratory infections. As is typical for environmental opportunistic pathogens, S. maltophilia has a large genome and a high degree of genetic diversity. In this study, we evaluated the genomic content of S. maltophilia, combining short and long read sequencing to construct nearly complete genomes of 10 clinical isolates. The genomes of these isolates were then compared with all publicly available S. maltophilia genome assemblies, and each isolate was then evaluated for colonization/persistence in vivo, both alone and in coinfection with P. aeruginosa. We found that while the overall genome size and GC content were fairly consistent between strains, there was considerable variability in both genome structure and gene content. Similarly, there was significant variability in S. maltophilia colonization and persistence in experimental mouse respiratory infections in the presence or absence of P. aeruginosa. Ultimately, this study gives us a greater understanding of the genomic diversity of clinical S. maltophilia isolates, and how this genomic diversity relates to both interactions with other pulmonary pathogens and to host disease progression. Identifying the molecular determinants of infection with S. maltophilia can facilitate development of novel antimicrobial strategies for a highly drug-resistant pathogen.
期刊介绍:
We publish high-quality original research on bacteria, fungi, protists, archaea, algae, parasites and other microscopic life forms.
Topics include but are not limited to:
Antimicrobials and antimicrobial resistance
Bacteriology and parasitology
Biochemistry and biophysics
Biofilms and biological systems
Biotechnology and bioremediation
Cell biology and signalling
Chemical biology
Cross-disciplinary work
Ecology and environmental microbiology
Food microbiology
Genetics
Host–microbe interactions
Microbial methods and techniques
Microscopy and imaging
Omics, including genomics, proteomics and metabolomics
Physiology and metabolism
Systems biology and synthetic biology
The microbiome.