S. Droog, N. Lakenberg, I. Meulenbelt, M.P.M. de Maat, L.G.M. Huisman, A.F.H. Jie, P.E. Slagboom
{"title":"群体研究中DNA的分离和储存","authors":"S. Droog, N. Lakenberg, I. Meulenbelt, M.P.M. de Maat, L.G.M. Huisman, A.F.H. Jie, P.E. Slagboom","doi":"10.1016/S0268-9499(96)80041-5","DOIUrl":null,"url":null,"abstract":"<div><p>For genetic population studies, human genomic DNA is commonly isolated from peripheral blood. A fast, non-invasive DNA sampling method is developed involving oral samples taken with cotton swabs. In addition various procedures were compared for isolation of DNA from different sources: whole blood or buffy-coats stored at −20°C for 5–10 years or buccal cells collected freshly with the non-invasive method. The differences in these procedures, which do not contain a phenol-extraction, are based on the use of either 1) high concentration ammonium acetate followed by DNA precipitation or 2) high concentration potassium acetate, followed by chloroform extraction and normal DNA precipitation.</p></div>","PeriodicalId":84750,"journal":{"name":"Fibrinolysis","volume":"10 ","pages":"Pages 29-30"},"PeriodicalIF":0.0000,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0268-9499(96)80041-5","citationCount":"6","resultStr":"{\"title\":\"Isolation and storage of DNA for population studies\",\"authors\":\"S. Droog, N. Lakenberg, I. Meulenbelt, M.P.M. de Maat, L.G.M. Huisman, A.F.H. Jie, P.E. Slagboom\",\"doi\":\"10.1016/S0268-9499(96)80041-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>For genetic population studies, human genomic DNA is commonly isolated from peripheral blood. A fast, non-invasive DNA sampling method is developed involving oral samples taken with cotton swabs. In addition various procedures were compared for isolation of DNA from different sources: whole blood or buffy-coats stored at −20°C for 5–10 years or buccal cells collected freshly with the non-invasive method. The differences in these procedures, which do not contain a phenol-extraction, are based on the use of either 1) high concentration ammonium acetate followed by DNA precipitation or 2) high concentration potassium acetate, followed by chloroform extraction and normal DNA precipitation.</p></div>\",\"PeriodicalId\":84750,\"journal\":{\"name\":\"Fibrinolysis\",\"volume\":\"10 \",\"pages\":\"Pages 29-30\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1996-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0268-9499(96)80041-5\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Fibrinolysis\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0268949996800415\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fibrinolysis","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0268949996800415","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Isolation and storage of DNA for population studies
For genetic population studies, human genomic DNA is commonly isolated from peripheral blood. A fast, non-invasive DNA sampling method is developed involving oral samples taken with cotton swabs. In addition various procedures were compared for isolation of DNA from different sources: whole blood or buffy-coats stored at −20°C for 5–10 years or buccal cells collected freshly with the non-invasive method. The differences in these procedures, which do not contain a phenol-extraction, are based on the use of either 1) high concentration ammonium acetate followed by DNA precipitation or 2) high concentration potassium acetate, followed by chloroform extraction and normal DNA precipitation.