{"title":"IgE-IV靶细胞受体的表征。","authors":"D.H. Conrad , A. Froese","doi":"10.1016/0161-5890(78)90086-X","DOIUrl":null,"url":null,"abstract":"<div><p>Complexes, consisting of IgE and its receptor from rat basophilic leukemia (RBL) cells, were isolated by allowing RBL cells to interact with DNP-IgE, solubilizing the cells with Nonidet P-40 and by allowing the extract to bind to anti-DNP-Sepharose. Of the RBL surface material eluted with 2,4-dinitrophenolate, 51–65% was precipitable by anti-IgE. Polyacrylamide gel analysis of eluates in SDS. using 10% gels, revealed a single surface component with an apparent molecular weight of about 45.000 daltons.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1978-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90086-X","citationCount":"8","resultStr":"{\"title\":\"Characterization of the target cell receptor for IgE—IV.\",\"authors\":\"D.H. Conrad , A. Froese\",\"doi\":\"10.1016/0161-5890(78)90086-X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Complexes, consisting of IgE and its receptor from rat basophilic leukemia (RBL) cells, were isolated by allowing RBL cells to interact with DNP-IgE, solubilizing the cells with Nonidet P-40 and by allowing the extract to bind to anti-DNP-Sepharose. Of the RBL surface material eluted with 2,4-dinitrophenolate, 51–65% was precipitable by anti-IgE. Polyacrylamide gel analysis of eluates in SDS. using 10% gels, revealed a single surface component with an apparent molecular weight of about 45.000 daltons.</p></div>\",\"PeriodicalId\":13265,\"journal\":{\"name\":\"Immunochemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1978-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0161-5890(78)90086-X\",\"citationCount\":\"8\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Immunochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/016158907890086X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/016158907890086X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Characterization of the target cell receptor for IgE—IV.
Complexes, consisting of IgE and its receptor from rat basophilic leukemia (RBL) cells, were isolated by allowing RBL cells to interact with DNP-IgE, solubilizing the cells with Nonidet P-40 and by allowing the extract to bind to anti-DNP-Sepharose. Of the RBL surface material eluted with 2,4-dinitrophenolate, 51–65% was precipitable by anti-IgE. Polyacrylamide gel analysis of eluates in SDS. using 10% gels, revealed a single surface component with an apparent molecular weight of about 45.000 daltons.
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