{"title":"球质体保存于- 70°C，转染φX174 RFDNA","authors":"H.V. Malling , M.C. Fater , J.G. Burkhart , S.C. Hardies , C.A. Hutchison III , M.H. Edgell","doi":"10.1016/0735-0651(87)90014-8","DOIUrl":null,"url":null,"abstract":"<div><p>A method was developed for preparation of spheroplasts used for transfection with φX174 RF DNA. This method had a high-level competence and retained the competence for up to one year of storage in 7% DMSO at −70°C.</p></div>","PeriodicalId":77714,"journal":{"name":"Gene analysis techniques","volume":"4 2","pages":"Pages 23-26"},"PeriodicalIF":0.0000,"publicationDate":"1987-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0735-0651(87)90014-8","citationCount":"4","resultStr":"{\"title\":\"Storage of spheroplasts at −70°C for transfection with φX174 RFDNA\",\"authors\":\"H.V. Malling , M.C. Fater , J.G. Burkhart , S.C. Hardies , C.A. Hutchison III , M.H. Edgell\",\"doi\":\"10.1016/0735-0651(87)90014-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A method was developed for preparation of spheroplasts used for transfection with φX174 RF DNA. This method had a high-level competence and retained the competence for up to one year of storage in 7% DMSO at −70°C.</p></div>\",\"PeriodicalId\":77714,\"journal\":{\"name\":\"Gene analysis techniques\",\"volume\":\"4 2\",\"pages\":\"Pages 23-26\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1987-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0735-0651(87)90014-8\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gene analysis techniques\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0735065187900148\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene analysis techniques","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0735065187900148","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Storage of spheroplasts at −70°C for transfection with φX174 RFDNA
A method was developed for preparation of spheroplasts used for transfection with φX174 RF DNA. This method had a high-level competence and retained the competence for up to one year of storage in 7% DMSO at −70°C.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
