大麦品种间缺氮生理性状及基因表达模式的比较分析。

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal, genetic engineering & biotechnology Pub Date : 2023-11-10 DOI:10.1186/s43141-023-00567-w
Zohreh Hajibarat, Abbas Saidi, Habibollah Ghazvini, Zahra Hajibarat
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引用次数: 0

摘要

背景:氮是植物最重要的矿物质营养素之一,主要以无机形式(NH+4和NO-3)被根系吸收。植物吸收氮作为生长、生物量生产和发育的食物来源。氮主要以硝酸盐的形式吸收,硝酸盐是高等植物最常见的氮来源。硝酸盐转运的一个独特特征是,NO-3在基因和生理水平上既是转运的底物,也是NO-3转运系统的诱导剂。方法:测定5个大麦品种对氮缺乏(ND)的形态和生理性状(叶绿素a/b、总叶绿素、类胡萝卜素、抗氧化酶和蛋白质含量)、性状与基因表达的相关性以及性状的主成分分析。将饥饿的植物转移到含有0.2mM和2mM NO-3的营养溶液中,分别在ND施用后7天和14天以及非胁迫条件下。结果:基因表达分析表明,在ND处理后7天和14天,5个大麦品种的叶片和根组织中都诱导了10个HvNRT2基因。通过对10个HvNRT2基因的相对定量qRT-PCR分析来测定NRT2基因表达。根据基因表达,HvNRT2.1、HvNRT2.2和HvNRT2.4被NO-3强烈诱导,在ND处理后7和14天达到峰值。相反,HvNRT2.4在叶片和根中都只表现出中等程度的诱导。从我们的结果来看,与非胁迫条件相比,热汗品种在ND处理后7天和14天的根系鲜重(RFW)、蛋白质含量和抗氧化酶活性显著增加。根系过氧化氢酶(CATr)与HvNRT2.2/2.5/2.6叶片呈高度正相关。结论:在长期缺氮过程中,HvNRT2.4的表达增加,而ND使HvNRT2.1和HvNRT2.2的表达短暂增加。根据生理、形态特征和分子机制,热汗被认为是ND条件下的耐受品种。
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Comparative analysis of physiological traits and gene expression patterns in nitrogen deficiency among barley cultivars.

Background: Nitrogen is one of the most important mineral nutrients for plants and is absorbed by the root system mainly in the inorganic form (NH+4 and NO-3). Plants absorb nitrogen as a food source for growth, biomass production, and development. Nitrogen is mainly absorbed as nitrate, which is the most common source of nitrogen available to higher plants. One of the unique features of nitrate transport is that NO-3 is both a substrate for transport and an inducer of NO-3 transport systems in genes and at physiological levels.

Methods: In the present study, morphological and physiological traits (chlorophyll a/b, total chlorophyll, and carotenoid, antioxidant enzymes, and protein content), correlation between traits and gene expression, and principle component analysis of traits among five barley cultivars were measured in response to nitrogen deficiency (ND). The starved plants were transferred to a nutrient solution containing 0.2 mM and 2 mM NO-3 up to 7 and 14 days after ND application and non-stressed conditions, respectively.

Results: Gene expression analysis revealed that the 10 HvNRT2 genes were induced in the leaf and root tissues at 7 and 14 days after ND treatments in five barley cultivars. Expression of NRT2 genes by relative quantitative qRT-PCR analysis for 10 HvNRT2 genes were determined. Based on the gene expression, HvNRT2.1, HvNRT2.2, and HvNRT2.4 were strongly induced by NO-3, peaking at 7 and 14 days after ND treatment. In contrast, the HvNRT2.4 showed only moderate induction in both leaves and roots. From our results, the Reyhan cultivar showed a significant increase in root fresh weight (RFW), protein content, and antioxidant enzyme activity in roots at 7 and 14 days after ND treatment as compared to the non-stressed condition. A highly positive correlation was observed between root catalase (CATr) and HvNRT2.2/2.5/2.6 leaves.

Conclusion: The expression of HvNRT2.4 is increased during long-term nitrogen starvation, while the expression of HvNRT2.1 and HvNRT2.2 are transiently increased by ND. Based on physiological and morphological traits and molecular mechanisms, the Reyhan is considered a tolerant cultivar under ND condition.

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