lix1在蜥蜴再生尾巴中的免疫定位表明该蛋白主要存在于神经组织中。

IF 2.3 4区生物学 Q4 CELL BIOLOGY Acta histochemica Pub Date : 2023-11-08 DOI:10.1016/j.acthis.2023.152113

Lorenzo Alibardi

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引用次数: 0

摘要

目的：蜥蜴的再生源于截肢后许多发育基因的重新激活。转录组显示，在表达最高的基因中，lix1的功能作用尚不清楚。方法：利用与蜥蜴Podarcis muralis lix1发生交叉反应的抗体，通过免疫荧光法检测再生尾巴中蛋白质的定位位点。结果：Lix1蛋白几乎完全定位于再生的脊髓（室管膜）和生长到芽基中的神经，在稀疏的芽基细胞中，但在其他组织中检测不到。结论：由于脊髓对刺激尾部再生至关重要，因此假设lix1蛋白是再生脊髓产生的信号传导或生长因子的一部分，这些因子是蜥蜴尾部再生所需的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Immunolocalization of lix1 in the regenerating tail of lizard indicates that the protein is mainly present in the nervous tissue

Purpose

Lizard regeneration derives from the re-activation of a number of developmental genes after tail amputation. Among genes with the highest expression, as indicated from the transcriptome, is lix1 which functional role is not known.

Method

An antibody that cross-reacts with the lizard Podarcis muralis lix1 has been utilized to detect by immunofluorescence the sites of localization of the protein in the regenerating tail.

Results

Lix1-protein is almost exclusively localized in the regenerating spinal cord (ependyma) and nerves growing into the blastema, in sparse blastema cells but is undetectable in other tissues.

Conclusions

Since the spinal cord is essential to stimulate tail regeneration it is hypothesized that the lix1 protein is part of the signaling or growing factors produced from the regenerating spinal cord that are needed for tail regeneration of the lizard tail.

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来源期刊

Acta histochemica 生物-细胞生物学

CiteScore

4.60

自引率

4.00%

发文量

107

审稿时长

23 days

期刊介绍： Acta histochemica, a journal of structural biochemistry of cells and tissues, publishes original research articles, short communications, reviews, letters to the editor, meeting reports and abstracts of meetings. The aim of the journal is to provide a forum for the cytochemical and histochemical research community in the life sciences, including cell biology, biotechnology, neurobiology, immunobiology, pathology, pharmacology, botany, zoology and environmental and toxicological research. The journal focuses on new developments in cytochemistry and histochemistry and their applications. Manuscripts reporting on studies of living cells and tissues are particularly welcome. Understanding the complexity of cells and tissues, i.e. their biocomplexity and biodiversity, is a major goal of the journal and reports on this topic are especially encouraged. Original research articles, short communications and reviews that report on new developments in cytochemistry and histochemistry are welcomed, especially when molecular biology is combined with the use of advanced microscopical techniques including image analysis and cytometry. Letters to the editor should comment or interpret previously published articles in the journal to trigger scientific discussions. Meeting reports are considered to be very important publications in the journal because they are excellent opportunities to present state-of-the-art overviews of fields in research where the developments are fast and hard to follow. Authors of meeting reports should consult the editors before writing a report. The editorial policy of the editors and the editorial board is rapid publication. Once a manuscript is received by one of the editors, an editorial decision about acceptance, revision or rejection will be taken within a month. It is the aim of the publishers to have a manuscript published within three months after the manuscript has been accepted