高脂饮食促进Sprague-Dawley大鼠凝血和内皮细胞活化:联合口服避孕药的短期效果。

IF 1.9 Q3 PERIPHERAL VASCULAR DISEASE Clinica e Investigacion en Arteriosclerosis Pub Date : 2024-03-01 DOI:10.1016/j.arteri.2023.10.001
Oyesanmi A. Fabunmi , Phiwayinkosi V. Dludla , Bongani B. Nkambule
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引用次数: 0

摘要

背景:联合口服避孕药(COCs)在个体中的使用与血栓形成事件的风险增加有关。这突出了评估COC对高脂肪饮食(HFD)喂养的Sprague-Dawley大鼠促进凝血和内皮激活的影响的重要性。方法:20只体重在150至200g之间的5周龄雌性Sprague-Dawley大鼠同时接受LFD和HFD喂养8周,以确定其对基础代谢状态、止血特性、血液动力学参数(血压和心率)的影响,以及凝血(组织因子和D-二聚体)和内皮激活(Von Willebrand因子和一氧化氮)的选定生物标志物。此后,HFD喂养的动物接受高剂量联合口服避孕药(HCOC)和低剂量联合口服避孕药具(LCOC)治疗6周。结果:与LFD组相比,除了体重增加外,HFD喂养还与高血糖、平均动脉压升高和一氧化氮水平降低有关(p0.05)。然而,本研究并非没有限制,因为这些标志物在这些动物的心脏组织或内皮细胞中的调节仍有待证实。结论:HFD喂养协调了大鼠促凝血因子和内皮激活标志物的同时释放,导致止血失衡和内皮功能障碍。COC的短期治疗在这些HFD喂养的大鼠中没有显示出有害影响。尽管就临床相关性而言,我们的研究结果表明,CVD与COC相关的风险可能取决于剂量和使用持续时间以及其他因素,尤其是在某些情况下。然而,还需要更多的研究来证实这些发现,特别是在与绝经后状态相关的某些条件下,这种治疗对这些动物的心脏组织或内皮细胞的长期影响。
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High-fat diet promotes coagulation and endothelial activation in Sprague Dawley rats: Short-term effects of combined oral contraceptives

Background

Combined oral contraceptives (COCs), use in individuals are associated with increased risk of thrombotic events. This highlights the significance of assessing the impact of COC on promoting coagulation and endothelial activation in high-fat diet (HFD)-fed Sprague Dawley rats.

Methods

Twenty (20) five-weeks-old female Sprague Dawley rats weighing between 150 and 200 g were subjected to both LFD and HFD-feeding for 8-weeks to determine its influence on basic metabolic status, hemostatic profile, hemodynamic parameters (blood pressure and heart rate), as well as selected biomarkers of coagulation (tissue factor and D-dimer) and endothelial activation (Von Willebrand factor and nitric oxide). Thereafter HFD-fed animals were treated with receive high dose combined oral contraceptive (HCOC) and low dose combine oral contraceptive (LCOC) for 6 weeks.

Results

Our results showed that beyond weight gain, HFD-feeding was associated with hyperglycemia, increased mean arterial pressure, and reduced nitric oxide levels when compared with LFD group (p < 0.05). Interestingly, treatment with high dose of COC for 6-weeks did not significantly alter atherothrombotic markers (p > 0.05). However, this study is not without limitation as regulation of these markers remains to be confirmed within the cardiac tissues or endothelial cells of these animals.

Conclusion

HFD-feeding orchestrate the concomitant release of pro-coagulants and endothelial activation markers in rats leading to haemostatic imbalance and endothelial dysfunction. Short-term treatment with COC shows no detrimental effects in these HFD-fed rats. Although in terms of clinical relevance, our findings depict the notion that the risk of CVD in association with COC may depend on the dosage and duration of use among other factors especially in certain conditions. However, additional studies are required to confirm these findings, especially long-term effects of this treatment within the cardiac tissues or endothelial cells of these animals in certain conditions relating to postmenopausal state.

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来源期刊
Clinica e Investigacion en Arteriosclerosis
Clinica e Investigacion en Arteriosclerosis PERIPHERAL VASCULAR DISEASE-
CiteScore
3.20
自引率
6.20%
发文量
44
审稿时长
40 days
期刊介绍: La publicación idónea para acceder tanto a los últimos originales de investigación como a formación médica continuada sobre la arteriosclerosis y su etiología, epidemiología, fisiopatología, diagnóstico y tratamiento. Además, es la publicación oficial de la Sociedad Española de Arteriosclerosis.
期刊最新文献
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