Ozonation of Bovine Peritoneal Membrane for Preservation: Preliminary Investigation

ABSTRACT A dynamic fluid system using ozonated water flowing over the bovine peritoneal membrane was developed. Histological analyses were performed to verify the maximum time of ozone exposure to maintain the viability of the biological material. The results indicate that the biological viability of the peritoneum tissue was impaired after 15 minutes of exposure (5.5 mg O3 (cm2)−1) to ozonated water in a dynamic condition. Thus, this biological sample can be submitted to the dynamic fluid system with ozonated water for 10 minutes at an applied ozone dose of 3.7 mg O3 (cm2)−1, without inducing significant changes to the tissue.