阴道感染念珠菌分离鉴定方法

aya ahmed mousa, A. Abdel-Rahman, M. Azab
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引用次数: 0

摘要

许多方法的抽样阴道假丝酵母菌的存在已经发展。这些技术在阴道念珠菌病的诊断和治疗中发挥着重要作用。过去,从阴道分离的念珠菌鉴定通常仅限于念珠菌属或白色念珠菌种。然而,由于认识到念珠菌种类在产生假定的毒力因子和对抗真菌药物的敏感性方面存在差异,因此更重视在物种水平上鉴定分离株。因此,一系列商业上可用的酵母鉴定系统现在可以与传统的鉴定程序结合使用。本研究采用沙巴鲁德葡萄糖琼脂培养基、显微鉴定、糖发酵试验和试管试验等多种表型鉴定方法对念珠菌进行了检测。聚合酶链反应(PCR)是一种快速、灵敏的基因分型技术。
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Identification methods of Candida isolated from vaginal infections
A number of methods of sampling the vagina for the presence of candida have been developed. Such techniques play an important role in the diagnosis and management of vaginal candidiasis. In the past, identification of candida isolated from the vagina has usually been limited to the genus Candida or to the species C. albicans. However, with the recognition that Candida species differ in the production of putative virulence factors and sensitivity to antifungal agents, greater emphasis has been placed on identification of isolates to species level. As a result, a range of commercially available systems for yeast identification can now be used in conjunction with traditional identification procedures. In this work, Candida detected by many phenotypic identification methods such as sabaroud dextrose agar medium, microscopical identification, sugar fermentation test and germ tube test. Polymerase chain reaction (PCR) also used as a fast and sensitive genotypic technic to identify Candida sp.
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