{"title":"嗜热菌锰过氧化氢酶基因的克隆及序列分析","authors":"Krisana Phucharoen, Yuuki Takenaka, T. Shinozawa","doi":"10.3109/10425170109084467","DOIUrl":null,"url":null,"abstract":"The manganese catalase gene (mnct) from Thermoleophilum album NM, a thermophilic bacterium, was cloned and its nucleotide sequence was analyzed. The gene consists of 885 bp (65.4% GC content) encoding 294 amino acids with a molecular mass of 32,500 Da. The deduced amino acid sequence shows similarities to those of Thermus species strain YS 8-13 (a thermophilic bacterium) and Bacillus halodurans (an alkaliphilic bacterium) with 61 and 54% identities, respectively.","PeriodicalId":11381,"journal":{"name":"DNA Sequence","volume":"113 1","pages":"413 - 417"},"PeriodicalIF":0.0000,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Molecular Cloning and Sequence Analysis of the Manganese Catalase Gene from Thermoleophilum album NM\",\"authors\":\"Krisana Phucharoen, Yuuki Takenaka, T. Shinozawa\",\"doi\":\"10.3109/10425170109084467\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The manganese catalase gene (mnct) from Thermoleophilum album NM, a thermophilic bacterium, was cloned and its nucleotide sequence was analyzed. The gene consists of 885 bp (65.4% GC content) encoding 294 amino acids with a molecular mass of 32,500 Da. The deduced amino acid sequence shows similarities to those of Thermus species strain YS 8-13 (a thermophilic bacterium) and Bacillus halodurans (an alkaliphilic bacterium) with 61 and 54% identities, respectively.\",\"PeriodicalId\":11381,\"journal\":{\"name\":\"DNA Sequence\",\"volume\":\"113 1\",\"pages\":\"413 - 417\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2001-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"DNA Sequence\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3109/10425170109084467\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"DNA Sequence","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/10425170109084467","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
摘要
从嗜热细菌Thermoleophilum album NM中克隆了锰过氧化氢酶基因(mnct)，并对其核苷酸序列进行了分析。该基因全长885 bp (GC含量65.4%)，编码294个氨基酸，分子量为32,500 Da。所得氨基酸序列与嗜热菌YS 8-13和嗜碱杆菌YS 8-13的氨基酸序列相似，同源性分别为61%和54%。
Molecular Cloning and Sequence Analysis of the Manganese Catalase Gene from Thermoleophilum album NM
The manganese catalase gene (mnct) from Thermoleophilum album NM, a thermophilic bacterium, was cloned and its nucleotide sequence was analyzed. The gene consists of 885 bp (65.4% GC content) encoding 294 amino acids with a molecular mass of 32,500 Da. The deduced amino acid sequence shows similarities to those of Thermus species strain YS 8-13 (a thermophilic bacterium) and Bacillus halodurans (an alkaliphilic bacterium) with 61 and 54% identities, respectively.
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