Chronobiological approach to study the physiological activity of Candida species

Rythmometric markers can be identified within a day during the study of biological characteristics in order to be used for differential diagnostics of pathogens of different patients’ physical condition. These principles are based on analysis of clinical isolates С. albicans, С. tropicalis and C. krusei allocated from the vaginal microbiota at Candida dysbiosis condition. Control examples were the master samples from the American Type Culture Collection (ATCC). Detailed research was conducted on physiological characteristics through the formation of biofilms by yeast pathogens. Biological activity of Candida sp. biofilming was observed within 2 days with 4 hours interval in winter. Daily cultures were used for the experiment to correspond to their maximum adhesion to the glass surface. It was important to obtain 6 measurements per day with 3-5 times repetition of experiment conditions during the specified timeline. In order to determine the periodicity of the parameters studied, the data was statistically processed by Student’s t-test, using Mann–Whitney criteria and nonparametric method of least square method.It was found out that biofilming activity during 24 hours (р < 0.05) of fungi exists and that all species have many principles in common. It was attested that the main rhythmometric parameters of diagnostic significance are the rhythm period and amplitude-phase stability. It was found that the daily dynamics of C. albicans 24433 biofilm formation from American Type Culture Collection was characterized by an ultradian (about 12-hours) contribution of the rhythm in the morning – 4 A.M and in the evening – 4 P.M. Significant circadian (approx. daily) rhythms of adhesion glass surface activity were revealed in C. non-albicans from American Type Culture Collection. The dynamic of biofilm formation isolates of yeast from female reproductive organs with Candida pathology was characterized by reliable ultradian (about 12-hour) harmonics which biological significance defines resistance to external impact and the ability to adaptively respond to periodic stimuli.To sum up, implementation of the chronobiological approach has opened up new prospects for studying the physiology of Сandida sp., as it enables us to predict the dynamics of microbial states and takes into account the specificity of emergency and long-term adaptation to different environmental factors. The detection of the circadian rhythm of biofilm formation activity of different Candida sp. strains provides the possibility to manage the vitality of the Society of Bacteria and Fungi and predicts its resistance to various antibiotics.