{"title":"用激光傅立叶全息显微镜进行三维衍射有限成像","authors":"V. Karpov","doi":"10.11648/J.IJGG.20180601.12","DOIUrl":null,"url":null,"abstract":"A laser holographic microscope (LHM) is investigated experimentally. The standard slide of Parascaris Univalens Iarva (ascaris) is studied. Comparison of the pictures of the same ascaris cell, observed by the LHM and high-performance Nikon conventional optical microscope (COM) 10×100/1.25 with immersion oil and green filter indicates that the both microscopes provide diffraction – limited 3-D spatial resolution, but dramatically different contrast. Thus, the LHM gives much more subcellular information.","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"2672 1","pages":"8"},"PeriodicalIF":0.0000,"publicationDate":"2018-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"3D Diffraction – Limited Imaging with a Laser Fourier Holographic Microscope\",\"authors\":\"V. Karpov\",\"doi\":\"10.11648/J.IJGG.20180601.12\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A laser holographic microscope (LHM) is investigated experimentally. The standard slide of Parascaris Univalens Iarva (ascaris) is studied. Comparison of the pictures of the same ascaris cell, observed by the LHM and high-performance Nikon conventional optical microscope (COM) 10×100/1.25 with immersion oil and green filter indicates that the both microscopes provide diffraction – limited 3-D spatial resolution, but dramatically different contrast. Thus, the LHM gives much more subcellular information.\",\"PeriodicalId\":88902,\"journal\":{\"name\":\"International journal of genetics and molecular biology\",\"volume\":\"2672 1\",\"pages\":\"8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-03-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of genetics and molecular biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.11648/J.IJGG.20180601.12\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of genetics and molecular biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.11648/J.IJGG.20180601.12","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
3D Diffraction – Limited Imaging with a Laser Fourier Holographic Microscope
A laser holographic microscope (LHM) is investigated experimentally. The standard slide of Parascaris Univalens Iarva (ascaris) is studied. Comparison of the pictures of the same ascaris cell, observed by the LHM and high-performance Nikon conventional optical microscope (COM) 10×100/1.25 with immersion oil and green filter indicates that the both microscopes provide diffraction – limited 3-D spatial resolution, but dramatically different contrast. Thus, the LHM gives much more subcellular information.
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