{"title":"氧化低密度脂蛋白预处理血小板对血小板诱导的血管松弛的衰减:血清素的重要作用","authors":"Baichun Yang, L. Chen, J. Mehta","doi":"10.3109/10623329609024702","DOIUrl":null,"url":null,"abstract":"Aggregating platelets cause relaxation of precontracted vascular tissues with intact endothelium. To determine the modulation of vasorelaxation by lipoprotein-rich platelets, rat platelets were preincubated with buffer, native low density lipoprotein (n-LDL, 100 μg protein/ml), oxidized LDL (ox-LDL, 100 μg protein/ml) or high density lipoprotein (HDL, 100 μg protein/ml) at 37°C for 1 hour, washed twice, and then suspended into organ baths containing precontracted endothelium-intact rat aortic rings. While all platelet preparations caused relaxation of rat aortic rings, the magnitude of relaxation induced by ox-LDL-treated platelets was less than that by buffer-treated platelets (p < 0.05). Pretreatment of platelets with native LDL or HDL did not affect the magnitude of vasorelaxation. Treatment of platelets with the cyclooxygenase inhibitor indomethacin (5 × 10−5 M) did not affect the attenuated vasorelaxation in response to ox-LDL-treated platelets. Pretreatment of aortic rings with the thromboxane (TX) ...","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"41 1","pages":"273-279"},"PeriodicalIF":0.0000,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Attenuation of Platelet-Induced Vasorelaxation by Pretreatment of Platelets with Oxidized Low Density Lipoproteins: Important Role of Serotonin\",\"authors\":\"Baichun Yang, L. Chen, J. Mehta\",\"doi\":\"10.3109/10623329609024702\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Aggregating platelets cause relaxation of precontracted vascular tissues with intact endothelium. To determine the modulation of vasorelaxation by lipoprotein-rich platelets, rat platelets were preincubated with buffer, native low density lipoprotein (n-LDL, 100 μg protein/ml), oxidized LDL (ox-LDL, 100 μg protein/ml) or high density lipoprotein (HDL, 100 μg protein/ml) at 37°C for 1 hour, washed twice, and then suspended into organ baths containing precontracted endothelium-intact rat aortic rings. While all platelet preparations caused relaxation of rat aortic rings, the magnitude of relaxation induced by ox-LDL-treated platelets was less than that by buffer-treated platelets (p < 0.05). Pretreatment of platelets with native LDL or HDL did not affect the magnitude of vasorelaxation. Treatment of platelets with the cyclooxygenase inhibitor indomethacin (5 × 10−5 M) did not affect the attenuated vasorelaxation in response to ox-LDL-treated platelets. Pretreatment of aortic rings with the thromboxane (TX) ...\",\"PeriodicalId\":11588,\"journal\":{\"name\":\"Endothelium-journal of Endothelial Cell Research\",\"volume\":\"41 1\",\"pages\":\"273-279\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1996-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Endothelium-journal of Endothelial Cell Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3109/10623329609024702\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Endothelium-journal of Endothelial Cell Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/10623329609024702","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Attenuation of Platelet-Induced Vasorelaxation by Pretreatment of Platelets with Oxidized Low Density Lipoproteins: Important Role of Serotonin
Aggregating platelets cause relaxation of precontracted vascular tissues with intact endothelium. To determine the modulation of vasorelaxation by lipoprotein-rich platelets, rat platelets were preincubated with buffer, native low density lipoprotein (n-LDL, 100 μg protein/ml), oxidized LDL (ox-LDL, 100 μg protein/ml) or high density lipoprotein (HDL, 100 μg protein/ml) at 37°C for 1 hour, washed twice, and then suspended into organ baths containing precontracted endothelium-intact rat aortic rings. While all platelet preparations caused relaxation of rat aortic rings, the magnitude of relaxation induced by ox-LDL-treated platelets was less than that by buffer-treated platelets (p < 0.05). Pretreatment of platelets with native LDL or HDL did not affect the magnitude of vasorelaxation. Treatment of platelets with the cyclooxygenase inhibitor indomethacin (5 × 10−5 M) did not affect the attenuated vasorelaxation in response to ox-LDL-treated platelets. Pretreatment of aortic rings with the thromboxane (TX) ...