Oligosaccharide Production from Agricultural Residues by Non-starch Polysaccharide Degrading Enzymes and Their Prebiotic Properties

Oligosaccharides were obtained from different agriculture residues by using non-starch polysaccharides (NSPs) degrading enzymes (Pentozyme®) hydrolysis method. Pentozyme consist of mixture of xylanase, amylase, β-glucanase, cellulase, mannanase, and pectinase. Agricultural residues including sugar palm peel, pine apple peel, spent tea leaves, spent coffee grounds, brewer's spent grain, copra meal, and rice straw were used as a source for oligosaccharide production. At the end of hydrolysis, reducing sugar and oligosaccharides content of all samples were measured. The results showed that reducing sugar content was significantly different (P<0.05) among the samples with spent tea leaves produced the highest reducing sugar and oligosaccharide after hydrolysis. Therefore, spent tea leaves were used in the subsequent experiment to evaluate its prebiotic properties. Results showed that the extract were able to inhibits growth of pathogen and support the growth of beneficial bacteria.