玉米(Zea mays L)低氮性状的三重杂交遗传分析

M. Afolabi, G. O. Agbowuro, A. Salami, M. Akoroda
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摘要

低氮玉米品种因其耐低氮土壤环境的能力而被培育出来。本试验旨在研究低氮玉米品种在低氮土壤条件下对籽粒产量及相关性状的遗传意义。本研究使用的自交系是由12个低氮耐受性开放授粉玉米品种自交6代后产生的。以2个自交系及其1代作为10个自交系的供试材料,采用三交杂交模式,产生30个杂交组合。在2017年的种植季节,30个杂交品种、它们的父母和测试者将送到州立大学阿多-埃基蒂分校。实验设计为随机完全区组设计(RCBD)。收集株高、穗高、开花期至50%、出丝期至50%、曲曲霉叶斑病发病率、枯萎病发病率、株向、穗向、穗腐病、留绿率、单株穗轴数、穗重、籽粒含水量和籽粒产量等数据。所有数据都经过方差分析和完整的遗传估计。各性状的加性和显性均显著(P < 0.05);然而,对于所有测试的性状,外延估计并不显著。显性度分量表明各性状均为偏显性。开花和出丝天数与株高、穗高、单株穗轴数、籽粒产量呈显著正相关(P < 0.05)。由于加性基因和显性基因的作用对低氮性状都很重要,因此在将低氮性状纳入玉米优良品种时,可以采用反向循环选择程序。
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Genetic analysis of low-n traits in maize (Zea mays L) using triple test cross
Low-N maize variety is developed for its ability to tolerate low nitrogen soil environment. This experiment was conducted to study the genetic implication of Low-N maize variety for grain yield and related traits under low nitrogen soil conditions triple test cross analysis. Inbred lines used for the study were generated from twelve low nitrogen tolerance open pollinated maize varieties after six generations of selfing. Two inbred lines along with their F1 were used as testers for ten inbred lines in a triple test cross pattern to generate 30 crosses. The 30 crosses, their parents and the testers to State University, Ado-Ekiti during 2017 planting season. The experimental design was a Randomized Complete Block Design (RCBD). Data were collected on plant height, ear height, days to 50% anthesis, days to 50% silking, incidence of curvularia leaf spot, blight, plant aspect, ear aspect, ear rot, stay green, cob per plant, ear weight, grain moisture content and grain yield. All data were subjected to analysis of variance and complete genetic estimates. Additive and dominants were significant (P < 0.05) for all traits; however, epitasis estimates were not significant for all the traits tested. The degree of dominance component indicated partial dominance for all the traits. Correlation coefficients for days to 50% anthesis and 50% silking, plant height, ear height, number of cobs per plant and grain yield were positive and significant (P < 0.05). Since both additive and dominance gene actions were important for low-N traits, the use of reciprocal recurrent selection procedure can be adopted in incorporating the trait into elite maize varieties.
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