肉鸡微生物负荷、耐药菌和抗生素残留

V. Kola, K. Srikanth, Shaik Muzammil Pasha, Y. S. Goutham, C. Pasha
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摘要

鸡肉是全世界最受欢迎的蛋白质食品。为了满足需求,建立了大型家禽养殖场,并使用抗生素预防和治疗细菌性疾病。不加控制地使用抗生素已导致家禽产生抗生素耐药性和家禽鸡产生抗生素残留。在多个零售点采集了51个鸡肉样本。采用高效液相色谱法测定抗生素残留量,利用细菌在培养基上的生长测定微生物总负荷,采用孔扩散法测定大肠杆菌、沙门氏菌、金黄色葡萄球菌和弯曲杆菌的耐药谱。除新霉素外,所有抗生素均在10-978 ppm范围内,平均微生物负荷在每克鸡样品7.32的log10范围内,大肠杆菌、沙门氏菌、金黄色葡萄球菌和弯曲杆菌对几种抗生素均有耐药性。因此,需要在家禽中适当使用抗生素,并在饲养和屠宰期间妥善处理鸡只。
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Microbial Load, Antibiotic Resistant Bacteria and Antibiotic Residues in Broiler Chicken
Chicken meat is highly preferred protein food worldwide. To meet the demand, huge poultry farms are established and using antibiotics as prophylaxis and treatment against the bacterial diseases. Uncontrolled usage of antibiotics has led to development of antibiotic resistance in poultry and antibiotic residues in poultry chicken. Fifty one chicken meat samples were collected from various retail outlets. Antibiotic residues were quantified by HPLC, total microbial load was measured by growth of bacteria on growth medium and antibiotic resistant profile of Escherichia coli, Salmonella spp, Staphylococcus aureus and Campylobacter spp was determined by well diffusion method. Except neomycin, all tested antibiotics were present in the range of 10-978 ppm, the average microbial load was in the range log 10 of 7.32 per gram of chicken sample, E. coli, Salmonella spp, Staphylococcus aureus and Campylobacter spp were resistant to several antibiotics studied. Hence there is a need of appropriate usage of antibiotics in poultry and proper handling of chicken during farming and slaughtering.
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