Leg muscle metabolism in trained and untrained men.

Abstract The purpose of this study was to describe substrate changes in blood and muscles during submaximal exercise and to look at the relationship of these changes to some enzyme activities important in lipid and carbohydrate metabolism. A group of six trained and six untrained men were exercised for 1 hour at 50% [Vdot]O2 max; three from each group cycled and the rest ran on a treadmill. During the exercise, blood-free fatty acid (FFA) levels increased twofold, glycerol by 3.6 times, triglyceride (TG) by 11/100 ml, and glucose by a mean 11 mg/100 ml in the trained men. In the untrained subjects, however, FFA increased 1.8 fold, glycerols 1.6 fold, and TG by a mean 24/100 ml. Glucose fell in every subject by an average 5 mg%. Muscle glycogen depletion averaged 34 mmoles/kg in the untrained and 23 mmoles/kg in the trained group. The activities of succinic acid dehydrogenase (SDH), malic acid dehydrogenase (MDH), and carnitine palmityl transferase (CPT) were greater by 177%, 93%, and 64%, respectively, in...