马来西亚诺氏疟原虫分裂子表面蛋白8的低水平多态性和负选择

Md Atique Ahmed, Hae-Ji Kang, F. Quan
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引用次数: 1

摘要

在大多数东南亚国家,特别是马来西亚,越来越多地报告了由于猴子疟疾寄生虫诺氏疟原虫引起的人类感染。这种寄生虫引起严重和致命的疟疾,因此需要采取紧急措施加以控制。本研究对马来西亚婆罗洲37份临床样本及6株实验室适应株的pkmsp8基因多态性、单倍型和自然选择水平进行了研究。该基因全长多态性水平较低,双表皮生长因子(EGF)结构域大多保守,不存在非同义替换。在非egf区域发现了强烈的负选择压力的证据,表明在不同的区域起作用的功能约束。系统发育单倍型网络分析确定了来自马来西亚半岛和马来西亚婆罗洲的共有单倍型,并指出了地理聚类。这是首次对马来西亚诺氏疟原虫临床分离株全长msp8基因进行遗传表征的研究;然而,进一步的功能表征将有助于未来合理的疫苗设计。
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Low Levels of Polymorphisms and Negative Selection in Plasmodum knowlesi Merozoite Surface Protein 8 in Malaysian Isolates
Human infections due to the monkey malaria parasite Plasmodium knowlesi is increasingly being reported from most Southeast Asian countries specifically Malaysia. The parasite causes severe and fatal malaria thus there is a need for urgent measures for its control. In this study, the level of polymorphisms, haplotypes and natural selection of full-length pkmsp8 in 37 clinical samples from Malaysian Borneo along with 6 lab-adapted strains were investigated. Low levels of polymorphism were observed across the full-length gene, the double epidermal growth factor (EGF) domains were mostly conserved, and non-synonymous substitutions were absent. Evidence of strong negative selection pressure in the non-EGF regions were found indicating functional constrains acting at different domains. Phylogenetic haplotype network analysis identified shared haplotypes and indicated geographical clustering of samples originating from Peninsular Malaysia and Malaysian Borneo. This is the first study to genetically characterize the full-length msp8 gene from clinical isolates of P. knowlesi from Malaysia; however, further functional characterization would be useful for future rational vaccine design.
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