临床样品中多重耐药大肠杆菌的分离与鉴定

Jahanara Utsad, C Prabhurajeshwar, C. Kelmani
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摘要

抗感染药物推荐方法的粗暴和卑劣导致临床样本中大肠杆菌分离株抗感染拮抗设计异常增加。该检查旨在通过传统和习惯方法从尿路污染患者的尿液检测中分离和识别多药物安全的尿路致病性大肠杆菌。从附近康复中心收治的患者收集了22份尿路感染样本。通过革兰氏再着色、吲哚、氧化酶、过氧化氢酶、甲基红、Voges-Proskauer、柠檬酸用量、溶血、运动性等生化试验来描述限制性菌株;明胶与尿素水解试验葡萄糖、乳糖和蔗糖的熟化和使用试验。针对一些习惯的和普通的抗毒素,解决了隔离的亲和性实例。从这次检查中发现56.75%的毒株为革兰氏阴性。95.23%的革兰氏阴性脱位观察吲哚能。吲哚阳性分离物对过氧化氢酶、甲基红、硝酸盐还原、运动、溶血、淀粉在汤培养基上成熟和淀粉在三倍糖琼脂上使用均有100%的肯定。氧化酶、Voges-Proskauer酶、脲酶、柠檬酸盐和明胶酶试验表现为玩世不恭。同样观察到,100%的吲哚阳性脱离在不同的培养基上给予灵感,例如,MacConkey琼脂和EMB琼脂。抗菌敏感性表明,所描绘的菌株100%是多药安全的。调查结果表明,鉴定出20株大肠杆菌,均为多药安全菌株。
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Isolation and Characterization of Multi Drug Resistant Escherichia Coli from Clinical Sample
Manhandle and despicable recommending approach of anti-infection agents causes exceptional increment of anti-infection opposition design among the E. coli isolates from clinical sample. This examination was directed to disconnect and recognize multi medicate safe uropathogenic Escherichia coli from urine test of urinary tract contaminated patients by tradition and customary methods. Twenty two (22) UTI urine samples were gathered from patients admitted to close-by healing centers. Portrayal of confined strains were dictated by biochemical tests like Gram recoloring, indole, oxidase, catalase, methyl red, Voges-Proskauer, citrate usage, hemolysis, motility; hydrolysis trial of gelatin and urea; maturation and use trial of glucose, lactose and sucrose. Affectability example of segregates was resolved against some customary and ordinary anti-toxins. From this examination, it was uncovered that 56.75% confines were gram negative. Indole energy was watched for 95.23% of Gram negative disengages. 100% of indole positive isolates were certain for catalase, methyl red, nitrate lessening, motility, and hemolysis, starch maturation on soup medium and starch use on Triple sugar press agar. Cynicism was seen for oxidase, Voges-Proskauer, urease, citrate, and gelatinase test. It was likewise watched that 100% of indole positive disengages gave inspiration on differential media, for example, MacConkey agar and EMB agar. Anti-microbial affectability shows 100% of portrayed strains were multi medicate safe. The discoveries of the investigation proposed that 20 E. coli were distinguished and every one of them were multi medicate safe strains.
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