组织工程中的干细胞——动态培养要求

D. Trišić, V. Jokanović, Đorđe Antonijević, D. Marković
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引用次数: 0

摘要

干细胞在体外组织工程、再生医学、细胞治疗和制药应用方面显示出巨大的潜力。所有这些应用,特别是在临床试验中,都需要有指导的高质量细胞的生产。传统的培养技术和应用已经在大多数原代和已建立的细胞系中进行,并对各种分析进行了标准化。然而,这些培养条件无法模拟干细胞在体内的动态和特化的三维微环境。为了提供干细胞体外生长的仿生微环境,三维培养技术已经被开发出来。在我们的研究中解释了新开发的多孔支架作为最有前途的体外模拟生态位的优势,它提供物理支持,使细胞生长、再生和新生血管形成,并及时被新形成的组织取代。此外,动态培养技术作为一种新的细胞培养方法,将是我们今后研究的重点。通过这种方式,通过开发一种最佳的动态培养方法,可以获得高质量的新细胞和组织,用于任何未来的临床应用。
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Stem cells in tissue engineering – dynamic cultivation requirement
Summary Stem cells have shown great potential for in vitro tissue engineering, regenerative medicine, cell therapy and pharmaceutical applications. All these applications, especially in clinical trials, will require guided production of high-quality cells. Traditional culture techniques and applications have been performed for the majority of primary and established cell lines and standardized for various analyses. Still, these culture conditions are unable to mimic dynamic and specialized three-dimensional microenvironment of the stem cells’ niche from in vivo conditions. In an attempt to provide biomimetic microenvironments for stem cells in vitro growth, three-dimensional culture techniques have been developed. In our study advantages of newly developed porous scaffolds as the most promising in vitro imitation of niche that provides physical support, enables cell growth, regeneration and neovascularization, while they are replaced in time with newly created tissue was explained. Furthermore, dynamic cultivation techniques have been described, as new way of cell culturing that will be the main subject of our future research. In that manner, by developing an optimal dynamic culturing method, high-quality new cells and tissues would be possible to obtain, for any future clinical application.
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