{"title":"由辣根过氧化物酶和金纳米粒子组成的过氧化氢纳米复合物的直接电子转移生物传感器-表征及其在双酶系统中的应用","authors":"Yusuke Okawa, Naoto Yokoyama, Yoshinori Sakai, Fumiyuki Shiba","doi":"10.1016/j.ancr.2015.05.001","DOIUrl":null,"url":null,"abstract":"<div><p>A reagentless electrochemical biosensor for hydrogen peroxide was fabricated. The sensor carries a monolayer of nanocomplex composed of horseradish peroxidase and Au-nanoparticle, and responds to hydrogen peroxide through the highly efficient direct electron transfer at a mild electrode potential without any soluble mediator. Formation of the nanocomplex was studied with visible spectroscopy and size exclusion chromatography. The sensor performance was analyzed based on a hydrodynamic electrochemical technique and enzyme kinetics. The sensor was applied to fabrication of sensors for glucose and uric acid through further modification of the nanocomplex-carrying electrode with the corresponding hydrogen peroxide-generating oxidases, glucose oxidase and urate oxidase, respectively.</p></div>","PeriodicalId":7819,"journal":{"name":"Analytical Chemistry Research","volume":"5 ","pages":"Pages 1-8"},"PeriodicalIF":0.0000,"publicationDate":"2015-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ancr.2015.05.001","citationCount":"20","resultStr":"{\"title\":\"Direct electron transfer biosensor for hydrogen peroxide carrying nanocomplex composed of horseradish peroxidase and Au-nanoparticle – Characterization and application to bienzyme systems\",\"authors\":\"Yusuke Okawa, Naoto Yokoyama, Yoshinori Sakai, Fumiyuki Shiba\",\"doi\":\"10.1016/j.ancr.2015.05.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A reagentless electrochemical biosensor for hydrogen peroxide was fabricated. The sensor carries a monolayer of nanocomplex composed of horseradish peroxidase and Au-nanoparticle, and responds to hydrogen peroxide through the highly efficient direct electron transfer at a mild electrode potential without any soluble mediator. Formation of the nanocomplex was studied with visible spectroscopy and size exclusion chromatography. The sensor performance was analyzed based on a hydrodynamic electrochemical technique and enzyme kinetics. The sensor was applied to fabrication of sensors for glucose and uric acid through further modification of the nanocomplex-carrying electrode with the corresponding hydrogen peroxide-generating oxidases, glucose oxidase and urate oxidase, respectively.</p></div>\",\"PeriodicalId\":7819,\"journal\":{\"name\":\"Analytical Chemistry Research\",\"volume\":\"5 \",\"pages\":\"Pages 1-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.ancr.2015.05.001\",\"citationCount\":\"20\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Chemistry Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2214181215000191\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry Research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214181215000191","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Direct electron transfer biosensor for hydrogen peroxide carrying nanocomplex composed of horseradish peroxidase and Au-nanoparticle – Characterization and application to bienzyme systems
A reagentless electrochemical biosensor for hydrogen peroxide was fabricated. The sensor carries a monolayer of nanocomplex composed of horseradish peroxidase and Au-nanoparticle, and responds to hydrogen peroxide through the highly efficient direct electron transfer at a mild electrode potential without any soluble mediator. Formation of the nanocomplex was studied with visible spectroscopy and size exclusion chromatography. The sensor performance was analyzed based on a hydrodynamic electrochemical technique and enzyme kinetics. The sensor was applied to fabrication of sensors for glucose and uric acid through further modification of the nanocomplex-carrying electrode with the corresponding hydrogen peroxide-generating oxidases, glucose oxidase and urate oxidase, respectively.