ReGeneraTing agents as matrix-based therapy loaded on gelatin/beta tricalcium phosphate sponge for the treatment of class II furcation defects in vitro

Objectives The experimental study was designed to evaluate the efficacy of gelatin sponge loaded with β tricalcium phosphate (β-TCP) with or without ReGeneraTing agent (RGTA) in the treatment of mandibular class II furcation defects in advanced periodontitis in vitro. Materials and methods Three Egyptian Baladi dogs were included with average age 13–16 months and average body weight of 9–10 kg. Six critical size defects class II furcation defects were created in the mandible of each dog, three in the right and three in the left mandibular second, third, and fourth premolars. All defects were made in the same way by the same operator. The defects were treated either with RGTA loaded on gelatin/β-TCP sponge or placebo loaded on gelatin sponge/β-TCP in two groups (group I and group II) that blindly received one of the treatment modalities. Samples were collected from the surgical sites of the experimental defects after 12 weeks. Results Histologically and histomorphometrically: there was a statistically significant difference when group I was compared with group II regarding surface area of new bone formation. Conclusion RGTA loaded on gelatin/β-TCP sponge could be considered as a potentiating material for periodontal regeneration.