{"title":"环吡唑酸诱导绵羊气管平滑肌收缩","authors":"S. Mustafa","doi":"10.1211/146080800128735511","DOIUrl":null,"url":null,"abstract":"The aim of this study was to determine the mechanism responsible for the cyclopiazonic acid (CPA)-induced contraction of tracheal smooth muscle. \n \n \n \nCPA, an inhibitor of Ca2+-ATPase, induced a slowly developing contraction of ovine tracheal strips but had no effect on bronchiolar ring segments. CPA-induced contractions in tracheal strips were abolished in Ca2+-free Krebs solution containing 2 mM EGTA, and were significantly reduced in the presence of verapamil. Influx of 45Ca2+ into the tracheal strips was significantly increased in the presence of CPA. \n \n \n \nThe results suggest that CPA induces contractions of ovine tracheal strips by promoting influx of extracellular Ca2+ through L-type voltage-dependent Ca2+ channels.","PeriodicalId":19946,"journal":{"name":"Pharmacy and Pharmacology Communications","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2000-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Cyclopiazonic Acid Induces Contractions in Ovine Tracheal Smooth Muscle\",\"authors\":\"S. Mustafa\",\"doi\":\"10.1211/146080800128735511\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The aim of this study was to determine the mechanism responsible for the cyclopiazonic acid (CPA)-induced contraction of tracheal smooth muscle. \\n \\n \\n \\nCPA, an inhibitor of Ca2+-ATPase, induced a slowly developing contraction of ovine tracheal strips but had no effect on bronchiolar ring segments. CPA-induced contractions in tracheal strips were abolished in Ca2+-free Krebs solution containing 2 mM EGTA, and were significantly reduced in the presence of verapamil. Influx of 45Ca2+ into the tracheal strips was significantly increased in the presence of CPA. \\n \\n \\n \\nThe results suggest that CPA induces contractions of ovine tracheal strips by promoting influx of extracellular Ca2+ through L-type voltage-dependent Ca2+ channels.\",\"PeriodicalId\":19946,\"journal\":{\"name\":\"Pharmacy and Pharmacology Communications\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2000-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmacy and Pharmacology Communications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1211/146080800128735511\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacy and Pharmacology Communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1211/146080800128735511","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
本研究的目的是确定环吡唑酸(CPA)诱导气管平滑肌收缩的机制。CPA是一种Ca2+- atp酶抑制剂，可诱导绵羊气管条缓慢收缩，但对细支气管环段无影响。在含有2 mM EGTA的无Ca2+ Krebs溶液中，cpa诱导的气管条收缩被消除，在维拉帕米的存在下，收缩明显减少。在CPA存在的情况下，45Ca2+流入气管条的量显著增加。结果表明，CPA通过l型电压依赖性Ca2+通道促进细胞外Ca2+的内流，从而诱导羊气管条收缩。
Cyclopiazonic Acid Induces Contractions in Ovine Tracheal Smooth Muscle
The aim of this study was to determine the mechanism responsible for the cyclopiazonic acid (CPA)-induced contraction of tracheal smooth muscle.
CPA, an inhibitor of Ca2+-ATPase, induced a slowly developing contraction of ovine tracheal strips but had no effect on bronchiolar ring segments. CPA-induced contractions in tracheal strips were abolished in Ca2+-free Krebs solution containing 2 mM EGTA, and were significantly reduced in the presence of verapamil. Influx of 45Ca2+ into the tracheal strips was significantly increased in the presence of CPA.
The results suggest that CPA induces contractions of ovine tracheal strips by promoting influx of extracellular Ca2+ through L-type voltage-dependent Ca2+ channels.
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