Miroslav Stredansky , Elena Conti , Claudia Bertocchi , Maria Matulova , Flavio Zanetti
{"title":"农杆菌产琥珀聚糖的研究","authors":"Miroslav Stredansky , Elena Conti , Claudia Bertocchi , Maria Matulova , Flavio Zanetti","doi":"10.1016/S0922-338X(98)80083-4","DOIUrl":null,"url":null,"abstract":"<div><p>A strain of <em>Agrobacterium tumefaciens</em> isolated from soil was cultivated under various conditions in shake flasks to study exopolysaccharides (EPS) production. NMR analysis revealed that the EPS obtained was a succinoglycan-like polymer. Optimal yields of EPS were obtained using sucrose and lysine as the carbon and nitrogen sources, respectively. Supplementation of the medium with various chemicals resulted in a more or less marked effect on the polymer yield and properties: in particular, high phosphate levels and non-ionic surfactants led to the production of polymers of different molecular sizes in yields up to 13.7 g/<em>l</em>. Oxygen availability also affected the polymer yield and quality. The chemical structure was substantially unaffected by the various fermentation conditions tested. A fermentation carried out in a laboratory-scale fermentor yielded 9.6 g/<em>l</em> succinoglycan from 15 g/<em>l</em> sucrose in the basic medium without further supplements.</p></div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 4","pages":"Pages 398-403"},"PeriodicalIF":0.0000,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80083-4","citationCount":"25","resultStr":"{\"title\":\"Succinoglycan production by Agrobacterium tumefaciens\",\"authors\":\"Miroslav Stredansky , Elena Conti , Claudia Bertocchi , Maria Matulova , Flavio Zanetti\",\"doi\":\"10.1016/S0922-338X(98)80083-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A strain of <em>Agrobacterium tumefaciens</em> isolated from soil was cultivated under various conditions in shake flasks to study exopolysaccharides (EPS) production. NMR analysis revealed that the EPS obtained was a succinoglycan-like polymer. Optimal yields of EPS were obtained using sucrose and lysine as the carbon and nitrogen sources, respectively. Supplementation of the medium with various chemicals resulted in a more or less marked effect on the polymer yield and properties: in particular, high phosphate levels and non-ionic surfactants led to the production of polymers of different molecular sizes in yields up to 13.7 g/<em>l</em>. Oxygen availability also affected the polymer yield and quality. The chemical structure was substantially unaffected by the various fermentation conditions tested. A fermentation carried out in a laboratory-scale fermentor yielded 9.6 g/<em>l</em> succinoglycan from 15 g/<em>l</em> sucrose in the basic medium without further supplements.</p></div>\",\"PeriodicalId\":15696,\"journal\":{\"name\":\"Journal of Fermentation and Bioengineering\",\"volume\":\"85 4\",\"pages\":\"Pages 398-403\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1998-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80083-4\",\"citationCount\":\"25\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Fermentation and Bioengineering\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0922338X98800834\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fermentation and Bioengineering","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0922338X98800834","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Succinoglycan production by Agrobacterium tumefaciens
A strain of Agrobacterium tumefaciens isolated from soil was cultivated under various conditions in shake flasks to study exopolysaccharides (EPS) production. NMR analysis revealed that the EPS obtained was a succinoglycan-like polymer. Optimal yields of EPS were obtained using sucrose and lysine as the carbon and nitrogen sources, respectively. Supplementation of the medium with various chemicals resulted in a more or less marked effect on the polymer yield and properties: in particular, high phosphate levels and non-ionic surfactants led to the production of polymers of different molecular sizes in yields up to 13.7 g/l. Oxygen availability also affected the polymer yield and quality. The chemical structure was substantially unaffected by the various fermentation conditions tested. A fermentation carried out in a laboratory-scale fermentor yielded 9.6 g/l succinoglycan from 15 g/l sucrose in the basic medium without further supplements.