杂交杨树基因型检测效率的载体研究

IF 1.1 4区 农林科学 Q3 FORESTRY Silvae Genetica Pub Date : 2021-01-01 DOI:10.2478/sg-2021-0004
N. Nelson, W. E. Berguson, B. McMahon, Meijun Cai, Daniel J. Buchman
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引用次数: 0

摘要

自然资源研究所杂交杨树项目为生物批量生产和环境服务培育和试验遗传改良无性系。测试过程从苗圃子代试验(NPT)到家庭田间试验(FFT)到克隆试验(CT)再到产量区(YB),在FFT和CT中对许多克隆进行有限的复制,并在单克隆区(YB)中进行有限数量的高度选择的克隆,以接近商业种植园的条件。我们使用相关向量R2(决定系数)和rs(斯皮尔曼系数)表示生长(DBH2),使用McFadden 's伪R2表示溃疡病严重程度评分,以确定哪些测试时间可以改变(年龄-年龄相关性)并消除整个测试步骤。FFT可以从5年缩短到4年。在CT中,5岁(半旋转)和9/10岁(全旋转)之间的等级相关性显著(R2 = 0.39 - 0.72),但在9/10岁时,5岁的选择错过了44%的前10个克隆。克隆在CT全旋转时的等级与YB全旋转时的等级相关,而不是半旋转时的等级。在CT中选择9岁的克隆增加了4年,但可以在克隆选择中消除YB。FFT和CT都是必要的。在早期不能确定全旋转CT上溃疡的丰度和严重程度。积极的策略可以节省6年的测试时间。
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Vectors of Efficiency in Hybrid Poplar Genotype Testing
Abstract The Natural Resources Research Institute Hybrid Poplar Program breeds and tests genetically improved clones for bio-mass production and environmental services. The testing process progresses from Nursery Progeny Tests (NPT) to Family Field Trials (FFT) to Clone Trials (CT) to Yield Blocks (YB), with limited replication of many clones in FFT and CT and a limited number of highly selected clones set out in monoclonal blocks (YB) to approximate the conditions of commercial plantations. We used correlation vectors, R2 (coefficient of determination) and rs (Spearman’s Coefficient) for growth (DBH2) and McFadden’s Pseudo R2 for canker severity score, to determine where testing times could be altered (age – age correlations) and whole testing steps eliminated. FFT can be shortened from 5 years to 4 years. In CT, rank correlations between age 5 (half-rotation) and age 9/10 (full rotation) were significant (R2 = 0.39 – 0.72), but age 5 selection missed 44 % of the top ten clones at age 9/10. Clone rank in CT at full, but not half, rotation was correlated with rank at full rotation in YB. Choosing clones at 9 years in CT adds 4 years but allows possible elimination of YB for clone selection. Both FFT and CT are necessary. Canker abundance and severity in CT at full rotation cannot be determined at earlier ages. An aggressive strategy saves 6 years of testing.
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来源期刊
Silvae Genetica
Silvae Genetica 农林科学-林学
CiteScore
2.20
自引率
10.00%
发文量
10
审稿时长
3 months
期刊介绍: Silvae Genetica is an international peer reviewed journal with more than 65 year tradition and experience in all fields of theoretical and applied Forest Genetics and Tree breeding. It continues "Zeitschrift für Forstgenetik und Forstpflanzenzüchtung" (Journal of Forest Genetics and Forest Tree Breeding) founded by W. LANGNER in 1951.
期刊最新文献
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