{"title":"冷活性微生物纤维素酶:了解其作用机理及其在食品饮料工业中的应用的新途径","authors":"G. Yunus, M. Kuddus","doi":"10.15414/JMBFS.2021.10.4.524-530","DOIUrl":null,"url":null,"abstract":"Cellulose, a carbohydrate mainly found in plant cell walls, is most abundant biopolymer on the Earth. Biodegradation of cellulose is carried out by a group of enzymes knows as cellulases. These enzymes play important roles in food processing and used in food industry. In the present era, cold-active enzymes are preferred instead of meso- and thermos-philic counterparts due to less energy requirement for their optimal activity and easy inactivation. The present study includes evaluation of cold-active cellulase from Pseudoalteromonas haloplanktis for its industrial applications in comparison to mesophilic and thermophilic cellulases, through molecular docking method. The binding energy of cold-active cellulase with the substrate cellulose was -126.60 KCals/mole. However, the energy for thermo- and mesophilic cellulase found to be -93.29 and -75.54 KCals/mole, respectively. The results concluded that cold-active cellulase has more efficacy compared to its counterparts and may be used in food processing industry at commercial level.","PeriodicalId":22746,"journal":{"name":"The Journal of Microbiology, Biotechnology and Food Sciences","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"COLD-ACTIVE MICROBIAL CELLULASE: NOVEL APPROACH TO UNDERSTAND MECHANISM AND ITS APPLICATIONS IN FOOD AND BEVERAGES INDUSTRY\",\"authors\":\"G. Yunus, M. Kuddus\",\"doi\":\"10.15414/JMBFS.2021.10.4.524-530\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Cellulose, a carbohydrate mainly found in plant cell walls, is most abundant biopolymer on the Earth. Biodegradation of cellulose is carried out by a group of enzymes knows as cellulases. These enzymes play important roles in food processing and used in food industry. In the present era, cold-active enzymes are preferred instead of meso- and thermos-philic counterparts due to less energy requirement for their optimal activity and easy inactivation. The present study includes evaluation of cold-active cellulase from Pseudoalteromonas haloplanktis for its industrial applications in comparison to mesophilic and thermophilic cellulases, through molecular docking method. The binding energy of cold-active cellulase with the substrate cellulose was -126.60 KCals/mole. However, the energy for thermo- and mesophilic cellulase found to be -93.29 and -75.54 KCals/mole, respectively. The results concluded that cold-active cellulase has more efficacy compared to its counterparts and may be used in food processing industry at commercial level.\",\"PeriodicalId\":22746,\"journal\":{\"name\":\"The Journal of Microbiology, Biotechnology and Food Sciences\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-01-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Journal of Microbiology, Biotechnology and Food Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15414/JMBFS.2021.10.4.524-530\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of Microbiology, Biotechnology and Food Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15414/JMBFS.2021.10.4.524-530","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
COLD-ACTIVE MICROBIAL CELLULASE: NOVEL APPROACH TO UNDERSTAND MECHANISM AND ITS APPLICATIONS IN FOOD AND BEVERAGES INDUSTRY
Cellulose, a carbohydrate mainly found in plant cell walls, is most abundant biopolymer on the Earth. Biodegradation of cellulose is carried out by a group of enzymes knows as cellulases. These enzymes play important roles in food processing and used in food industry. In the present era, cold-active enzymes are preferred instead of meso- and thermos-philic counterparts due to less energy requirement for their optimal activity and easy inactivation. The present study includes evaluation of cold-active cellulase from Pseudoalteromonas haloplanktis for its industrial applications in comparison to mesophilic and thermophilic cellulases, through molecular docking method. The binding energy of cold-active cellulase with the substrate cellulose was -126.60 KCals/mole. However, the energy for thermo- and mesophilic cellulase found to be -93.29 and -75.54 KCals/mole, respectively. The results concluded that cold-active cellulase has more efficacy compared to its counterparts and may be used in food processing industry at commercial level.