水稻核糖体RNA基因的甲基化模式

Adela Olmedilla , Dominique Delcasso, Michel Delseny
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引用次数: 17

摘要

建立了水稻(Oryza sativa) rDNA的EcoRI、BamHI和Bg1II限制性图谱。利用hpai和MspI研究了这些基因的甲基化模式。令人惊讶的是,水稻的rDNA比目前研究的其他植物的rDNA对这些酶更敏感。在有氧和无氧条件下生长的胚胎制备的rDNA甲基化模式没有差异,尽管在这两种情况下rRNA合成速率存在显著差异。
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Methylation pattern of nuclear ribosomal RNA genes from rice (Oryza sativa)

A restriction map of rice (Oryza sativa) rDNA was established for EcoRI, BamHI and Bg1II. The methylation pattern of these genes was studied using HpaII and MspI. Surprisingly, rice rDNA was found much more sensitive to these enzymes than the rDNA of the other plant species studied so far. No difference was observed between the methylation pattern of rDNA prepared from embryos grown in aerobic or anaerobic conditions although there is a marked difference between the rates of rRNA synthesis in these two situations.

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