{"title":"氧对丁酸梭菌(梭菌属模式种)生长的影响及梭菌体内氧酶和活性氧酶的分布","authors":"Sinji Kawasaki , Tomoyuki Nakagawa , Yoshitaka Nishiyama , Yoshimi Benno , Tai Uchimura , Kazuo Komagata , Michio Kozaki , Youichi Niimura","doi":"10.1016/S0922-338X(99)89006-0","DOIUrl":null,"url":null,"abstract":"<div><p>Clostridia are well-known obligatory anaerobic bacteria which cannot utilize oxygen, or otherwise die in oxygenated environments. <em>Clostridium butyricum</em>, the type species of the genus <em>Clostridium</em>, possesses the ability to consume oxygen in amounts proportional to the size of the inoculum. Oxygen consumption was observed when NADH and NADPH were added to the cell extract of this strain. NADH oxidase and NADPH oxidase activities were also detected in all of the tested strains of the genus <em>Clostridium. C. butyricum</em> ceased growing while consuming oxygen in the medium. After consumption of all the dissolved oxygen, <em>C. butyricum</em> resumed growth at a rate equivalent to its anaerobic growth rate, suggesting that no oxidative damage based on oxygen reduction occurs <em>in vivo</em>. As scavengers for active oxygen species, the activities of <span><math><mtext>NADH</mtext><mtext>NADPH</mtext></math></span> peroxidase and SOD were detected in <em>C. butyricum</em>. Furthermore, the activities of these enzymes are distributed widely in the genus <em>Clostridium</em>.</p></div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 4","pages":"Pages 368-372"},"PeriodicalIF":0.0000,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(99)89006-0","citationCount":"56","resultStr":"{\"title\":\"Effect of oxygen on the growth of Clostridium butyricum (type species of the genus Clostridium), and the distribution of enzymes for oxygen and for active oxygen species in Clostridia\",\"authors\":\"Sinji Kawasaki , Tomoyuki Nakagawa , Yoshitaka Nishiyama , Yoshimi Benno , Tai Uchimura , Kazuo Komagata , Michio Kozaki , Youichi Niimura\",\"doi\":\"10.1016/S0922-338X(99)89006-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Clostridia are well-known obligatory anaerobic bacteria which cannot utilize oxygen, or otherwise die in oxygenated environments. <em>Clostridium butyricum</em>, the type species of the genus <em>Clostridium</em>, possesses the ability to consume oxygen in amounts proportional to the size of the inoculum. Oxygen consumption was observed when NADH and NADPH were added to the cell extract of this strain. NADH oxidase and NADPH oxidase activities were also detected in all of the tested strains of the genus <em>Clostridium. C. butyricum</em> ceased growing while consuming oxygen in the medium. After consumption of all the dissolved oxygen, <em>C. butyricum</em> resumed growth at a rate equivalent to its anaerobic growth rate, suggesting that no oxidative damage based on oxygen reduction occurs <em>in vivo</em>. As scavengers for active oxygen species, the activities of <span><math><mtext>NADH</mtext><mtext>NADPH</mtext></math></span> peroxidase and SOD were detected in <em>C. butyricum</em>. Furthermore, the activities of these enzymes are distributed widely in the genus <em>Clostridium</em>.</p></div>\",\"PeriodicalId\":15696,\"journal\":{\"name\":\"Journal of Fermentation and Bioengineering\",\"volume\":\"86 4\",\"pages\":\"Pages 368-372\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1998-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0922-338X(99)89006-0\",\"citationCount\":\"56\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Fermentation and Bioengineering\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0922338X99890060\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fermentation and Bioengineering","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0922338X99890060","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Effect of oxygen on the growth of Clostridium butyricum (type species of the genus Clostridium), and the distribution of enzymes for oxygen and for active oxygen species in Clostridia
Clostridia are well-known obligatory anaerobic bacteria which cannot utilize oxygen, or otherwise die in oxygenated environments. Clostridium butyricum, the type species of the genus Clostridium, possesses the ability to consume oxygen in amounts proportional to the size of the inoculum. Oxygen consumption was observed when NADH and NADPH were added to the cell extract of this strain. NADH oxidase and NADPH oxidase activities were also detected in all of the tested strains of the genus Clostridium. C. butyricum ceased growing while consuming oxygen in the medium. After consumption of all the dissolved oxygen, C. butyricum resumed growth at a rate equivalent to its anaerobic growth rate, suggesting that no oxidative damage based on oxygen reduction occurs in vivo. As scavengers for active oxygen species, the activities of peroxidase and SOD were detected in C. butyricum. Furthermore, the activities of these enzymes are distributed widely in the genus Clostridium.