龙葵原生质体愈伤组织形成过程中细胞核dna含量的变化

Plant Science Letters Pub Date : 1984-07-01 DOI:10.1016/0304-4211(84)90233-5

Irene Carlberg , Kristina Glimelius , Tage Eriksson

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引用次数: 49

摘要

用碘化丙啶作为荧光染料，对马铃薯原生质体愈伤组织形成过程中细胞核dna含量进行了细胞荧光测定。在消化细胞壁和RNase处理后获得了可重复的测量结果。高dna含量的细胞核在培养早期就被发现，随着培养时间的延长，这些细胞核出现的频率增加。除了高度复制的细胞核外，还发现了多核细胞，随着培养时间的延长，多核细胞的出现频率降低。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Nuclear DNA-content during the initiation of callus formation from isolated protoplasts of Solanum tuberosum L

Cytofluorimetric measurements of the DNA-content in the nuclei during the initiation of callus formation from isolated protoplasts of potato were performed using propidium iodide as fluorochrome. Reproducible measurements were obtained after digestion of the cell walls and after RNase treatment.

Nuclei with high DNA-content were found early in the culture and the frequency of these nuclei increased with time in culture. In addition to highly replicated nuclei, multinucleated cells were found, the frequency of which decreased during prolonged culturing.

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