急性l -谷氨酰胺剥夺影响U87胶质瘤细胞中tp53相关蛋白基因的表达。

D. Minchenko, S. V. Danilovskyi, I. V. Kryvdiuk, N. A. Hlushchak, O. V. Kovalevska, L. L. Karbovskyi, O. Minchenko
{"title":"急性l -谷氨酰胺剥夺影响U87胶质瘤细胞中tp53相关蛋白基因的表达。","authors":"D. Minchenko, S. V. Danilovskyi, I. V. Kryvdiuk, N. A. Hlushchak, O. V. Kovalevska, L. L. Karbovskyi, O. Minchenko","doi":"10.15407/FZ60.04.011","DOIUrl":null,"url":null,"abstract":"We have studied the effect of acute L-glutamine deprivation on the expression oftumor protein 53 (TP53)-related genes such as TOPORS (topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase), TP53BPI (TP53 bindingprotein 1), TP53TG1 (TP53 inducible gene 1), SESN1 (p53 regulatedPA26 nuclear protein), NME6 (NME/NM23 nucleoside diphosphate kinase 6), and ZMAT3 (zinc finger Matrin-type 3) in glioma cells with ERN1 knockdown. It was shown that blockade of ERN1 finction in U87 glioma cells is induced the expression of RYBP and SESN1 genes, but decreased the expression of TP53BP1, TP53TG1, TOPORS, NME6, genes. Moreover, the expression levels ofRYBPI SESN1, TP53BP1, and ZMAT3 genes is increased in control glioma cells by L-glutamine deprivation condition but blockade of ERN1 signaling enzyme function significantly enhances this effect on the expression all of these genes. At the same time, the ERN1 knockdown eliminates the response TP53TG1 and TOPORS genes to L-glutamine deprivation condition. Results of this investigation clearly demonstrate that the expression most of genes encoding TP53-related factors depends upon acute L-glutamine deprivation condition as well as upon ERN1, the major signaling system of the endoplasmic reticulum stress.","PeriodicalId":12306,"journal":{"name":"Fiziolohichnyi zhurnal","volume":"8 1","pages":"11-21"},"PeriodicalIF":0.0000,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"9","resultStr":"{\"title\":\"Acute L-glutamine deprivation affects the expression of TP53-related protein genes in U87 glioma cells.\",\"authors\":\"D. Minchenko, S. V. Danilovskyi, I. V. Kryvdiuk, N. A. Hlushchak, O. V. Kovalevska, L. L. Karbovskyi, O. Minchenko\",\"doi\":\"10.15407/FZ60.04.011\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We have studied the effect of acute L-glutamine deprivation on the expression oftumor protein 53 (TP53)-related genes such as TOPORS (topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase), TP53BPI (TP53 bindingprotein 1), TP53TG1 (TP53 inducible gene 1), SESN1 (p53 regulatedPA26 nuclear protein), NME6 (NME/NM23 nucleoside diphosphate kinase 6), and ZMAT3 (zinc finger Matrin-type 3) in glioma cells with ERN1 knockdown. It was shown that blockade of ERN1 finction in U87 glioma cells is induced the expression of RYBP and SESN1 genes, but decreased the expression of TP53BP1, TP53TG1, TOPORS, NME6, genes. Moreover, the expression levels ofRYBPI SESN1, TP53BP1, and ZMAT3 genes is increased in control glioma cells by L-glutamine deprivation condition but blockade of ERN1 signaling enzyme function significantly enhances this effect on the expression all of these genes. At the same time, the ERN1 knockdown eliminates the response TP53TG1 and TOPORS genes to L-glutamine deprivation condition. Results of this investigation clearly demonstrate that the expression most of genes encoding TP53-related factors depends upon acute L-glutamine deprivation condition as well as upon ERN1, the major signaling system of the endoplasmic reticulum stress.\",\"PeriodicalId\":12306,\"journal\":{\"name\":\"Fiziolohichnyi zhurnal\",\"volume\":\"8 1\",\"pages\":\"11-21\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2014-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"9\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Fiziolohichnyi zhurnal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15407/FZ60.04.011\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fiziolohichnyi zhurnal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15407/FZ60.04.011","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 9

摘要

我们研究了急性l -谷氨酰胺剥夺对肿瘤蛋白53 (TP53)相关基因如TOPORS(拓扑异构酶I结合、富含精氨酸/丝氨酸、E3泛素蛋白连接酶)、TP53BPI (TP53结合蛋白1)、TP53TG1 (TP53诱导基因1)、SESN1 (p53调节的pa26核蛋白)、NME6 (NME/NM23核苷二磷酸激酶6)和ZMAT3(锌指基质型3)在ERN1敲低的胶质瘤细胞中的表达的影响。结果表明,在U87胶质瘤细胞中,阻断ERN1功能可诱导RYBP和SESN1基因的表达,而抑制TP53BP1、TP53TG1、TOPORS、NME6等基因的表达。此外,在l -谷氨酰胺剥夺条件下,rybpi SESN1、TP53BP1和ZMAT3基因的表达水平在对照胶质瘤细胞中增加,但阻断ERN1信号酶功能可显著增强对所有这些基因表达的影响。同时,ERN1敲低消除了TP53TG1和TOPORS基因对l -谷氨酰胺剥夺条件的反应。本研究结果清楚地表明,大多数编码tp53相关因子的基因的表达依赖于急性l -谷氨酰胺剥夺状态以及内质网应激的主要信号系统ERN1。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Acute L-glutamine deprivation affects the expression of TP53-related protein genes in U87 glioma cells.
We have studied the effect of acute L-glutamine deprivation on the expression oftumor protein 53 (TP53)-related genes such as TOPORS (topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase), TP53BPI (TP53 bindingprotein 1), TP53TG1 (TP53 inducible gene 1), SESN1 (p53 regulatedPA26 nuclear protein), NME6 (NME/NM23 nucleoside diphosphate kinase 6), and ZMAT3 (zinc finger Matrin-type 3) in glioma cells with ERN1 knockdown. It was shown that blockade of ERN1 finction in U87 glioma cells is induced the expression of RYBP and SESN1 genes, but decreased the expression of TP53BP1, TP53TG1, TOPORS, NME6, genes. Moreover, the expression levels ofRYBPI SESN1, TP53BP1, and ZMAT3 genes is increased in control glioma cells by L-glutamine deprivation condition but blockade of ERN1 signaling enzyme function significantly enhances this effect on the expression all of these genes. At the same time, the ERN1 knockdown eliminates the response TP53TG1 and TOPORS genes to L-glutamine deprivation condition. Results of this investigation clearly demonstrate that the expression most of genes encoding TP53-related factors depends upon acute L-glutamine deprivation condition as well as upon ERN1, the major signaling system of the endoplasmic reticulum stress.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
COMPARISON OF SPECTROSCOPIC PROPERTIES OF INTRAOCULAR FLUID IN PATIENTS WITH CATARACT AND PRIMARY OPEN-ANGLE GLAUCOMA. Experimental therapy of graft-versus-host disease by mesenchymal stromal cells grown on oxide nanocoatings. THE EFFECT OF GADOLINIUM ORTHOVANADATE NANOPARTICLES BY NEONATAL INDUCED REPRODUCTIVE DISEASE IN MALE RATS. Role of matrix metalloproteinase 9 and its tissue inhibitor 1 in development and prognosis of diabetic retinopathy. SPECIFIC FEATURES OF CHRONORHYTH- MOLOGIC CHANGES OF THE ION-REGU- LATING FUNCTION OF THE KIDNEYS UN- DER THE HYPOFUNCTION OF THE PINEAL GLAND.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1