Duong Duc Hoang Sinh, Nguyen Hoang Tue, T. Thi, Le Dang Thi Thanh, N. D. Huy, N. Loc
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引用次数: 0
摘要
联苯2,3-双加氧酶(Biphenyl 2,3-dioxygenase, BphA)是一种在细菌体内参与联苯降解途径起始步骤的酶。联苯是一种含有两个苯环的芳香烃，对环境造成严重污染。本文报道了从越南二恶英污染土壤中分离的洋葱芽孢杆菌DF2和DF4菌株BphA基因的克隆和表达的初步结果。BphA基因的编码DNA序列长度为897 bp，同源性为99%。重组BphA酶(33 kDa)经0.5 mM IPTG在37℃下诱导4 h后，在大肠杆菌M15中得到了强表达。
HETEROLOGOUS EXPRESSION OF A GENE ENCODING BIPHENYL 2,3-DIOXYGENASE FROM BURKHOLDERIA CEPACIA IN ESCHERICHIA COLI
Biphenyl 2,3-dioxygenase (BphA) is an enzyme that participates in initiation step of the pathway of biphenyl degradation in bacteria. Biphenyl is an aromatic hydrocarbon including two benzene rings that causes a serious environmental pollution. This report presents the preliminary results on the cloning and expression of BphA gene of B. cepacia strains DF2 and DF4 which were isolated from dioxin contaminated soil in Vietnam. The coding DNA sequences of BphA genes are 897 bp long and 99% identical. Recombinant BphA enzyme (33 kDa) has strongly expressed in E. coli M15 after induction of 0.5 mM IPTG for 4 h at 37oC.
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