铜绿假单胞菌PAO1中RecR的晶体结构

S. Che, Yujing Chen, Yakun Liang, Qionglin Zhang, M. Bartlam
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引用次数: 0

摘要

DNA损伤通常对所有生物体都是致命的。同源重组在原核生物DNA损伤修复过程中起着重要作用。铜绿假单胞菌同源重组有两种途径:RecBCD途径和RecFOR途径。RecR是铜绿假单胞菌RecFOR同源重组途径的重要调控因子。它与RecF和RecO形成复合物,可以促进RecA在RecFOR途径中装载到ssDNA上。本文报道了P. aeruginosa PAO1 (PaRecR)中RecR的晶体结构。PaRecR在P6122空间群中结晶,每个不对称单元有两个单体。分析超离心数据表明,PaRecR形成稳定的二聚体,但在溶液中可以作为四聚体存在。晶体结构表明,二聚体parrecr通过晶体对称形成环状四聚体结构。在一个RecR亚基的Walker B基序中存在配体表明可能存在核苷酸结合位点。
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Crystal structure of RecR from Pseudomonas aeruginosa PAO1
DNA damage is usually lethal to all organisms. Homologous recombination plays an important role in the DNA damage-repair process in prokaryotic organisms. Two pathways are responsible for homologous recombination in Pseudomonas aeruginosa: the RecBCD pathway and the RecFOR pathway. RecR is an important regulator in the RecFOR homologous recombination pathway in P. aeruginosa. It forms complexes with RecF and RecO that can facilitate the loading of RecA onto ssDNA in the RecFOR pathway. Here, the crystal structure of RecR from P. aeruginosa PAO1 (PaRecR) is reported. PaRecR crystallizes in space group P6122, with two monomers per asymmetric unit. Analytical ultracentrifugation data show that PaRecR forms a stable dimer, but can exist as a tetramer in solution. The crystal structure shows that dimeric PaRecR forms a ring-like tetramer architecture via crystal symmetry. The presence of a ligand in the Walker B motif of one RecR subunit suggests a putative nucleotide-binding site.
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期刊介绍: Acta Crystallographica Section F is a rapid structural biology communications journal. Articles on any aspect of structural biology, including structures determined using high-throughput methods or from iterative studies such as those used in the pharmaceutical industry, are welcomed by the journal. The journal offers the option of open access, and all communications benefit from unlimited free use of colour illustrations and no page charges. Authors are encouraged to submit multimedia content for publication with their articles. Acta Cryst. F has a dedicated online tool called publBio that is designed to make the preparation and submission of articles easier for authors.
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