H2O2和NO通过调节两种基因型茄子抗氧化酶来缓解盐胁迫

Fahriye ÖCAL ÖZDAMAR, Gökçen Baysal Furtana, R. Tıpırdamaz, H. Duman
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摘要

h2o2和NO是植物信号传导和感知的关键分子。在本研究中,我们针对叶施茄子的抗氧化能力进行了研究,这些基因型对盐度有不同的响应(Artvin:盐敏感;马尔丁省:耐盐)。为此,在幼苗叶片上喷洒h2o2和NO供体(SNP) 2 d,然后在100 mM NaCl中处理10 d。丙二醛(MDA)是脂质过氧化最重要的指标,随着盐的施用而增加,而单独或联合处理h2o2和NO供体显著降低。供体叶面喷施对SOD和CAT酶活性有影响。两种基因型的CAT酶活性均随着盐的施用而显著增加,但在单独或联合施用供体时,CAT酶活性再次显著增加。另一方面,两种基因型的SOD酶活性在盐胁迫处理下均有轻微升高,而在单独或共同施用盐胁迫下均有显著升高。
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H2O2 and NO mitigate salt stress by regulating antioxidant enzymes in in two eggplant (Solanum melongena L.) genotypes
H 2 O 2 and NO are the key molecules of plant signaling and perception. In this study, we aimed at the antioxidant capacity of foliar-applied eggplant genotypes which shows different responses to salinity (Artvin: salt-sensitive; Mardin: salt-tolerant). For this purpose, H 2 O 2 and NO donor (SNP) were sprayed on the leaves of the seedlings for 2 days and then exposed to 100 mM NaCl for 10 days. The amount of Malondialdehyde (MDA), which increased with salt application and is the most important indicator of lipid peroxidation, decreased significantly with individual or combined pretreatments of H 2 O 2 and NO donors. SOD and CAT enzyme activities are affected by foliar spraying of donors. While CAT enzyme activity increased significantly with salt application in both genotypes, it showed a significant increase again with individual or combined application of donors. SOD enzyme activity, on the other hand, showed a minor increase in both genotypes with the application of salt stress, while it was significantly increased with the application of donors individually or together.
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