不同持续时间的神经性糖尿病足溃疡中的生长因子及其受体

IF 0.7 Q4 ENDOCRINOLOGY & METABOLISM Diabetes Mellitus Pub Date : 2023-07-05 DOI:10.14341/dm13009
© Е.Ю. Комелягина, М. Б. Анциферов, Е.А. Коган, Н. В. Жарков, Elena Y. Komelyagina, Mikhail B. Antsiferov, Eugenia A. Kogan, Nikolay V. Zharkov
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Meanwhile, the results obtained will help to expand the understanding of the pathogenetic mechanisms of the course and outcomes of diabetic foot ulcers at different stages of healing.AIM: To evaluate the level of growth factors and their receptors in non-healing neuropathic diabetic foot ulcers of different duration.MATERIALS AND METHODS. 89 samples of neuropathic diabetic foot ulcers were examined. Immunohistochemical analysis of biopsies was performed using antibodies to the following growth factors and their receptors: vascular endothelial growth factor (VEGF), insulin-like growth factor (IGF), epidermal growth factor (EGF). Depending on the duration of the wound, the biopsies were divided into 3 groups: group 1 — ≤ 90 days, group 2 — 91-365 days, group 3 — > 365 days.RESULTS. In the samples of wounds, the duration of which did not exceed 90 days, the expression of growth factors and receptors to them was significantly higher than in wounds of longer duration (p=0.0001). 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引用次数: 0

摘要

背景。生长因子在伤口愈合中起主导作用。它们在糖尿病足综合征中的缺乏已在实验研究中得到证实,其中大多数是在急性伤口上进行的。同时,目前对这类患者局部使用生长因子治疗的有效性、适应证和禁忌症存在矛盾,是一个有待探讨的问题。没有关于不同持续时间的糖尿病足溃疡中生长因子含量的数据。同时,所获得的结果将有助于扩大对糖尿病足溃疡在不同愈合阶段的病程和结局的发病机制的理解。目的:探讨不同病程的未愈合神经性糖尿病足溃疡组织中生长因子及其受体的水平。材料和方法。对89例神经性糖尿病足溃疡患者进行了检查。使用以下生长因子及其受体的抗体对活检组织进行免疫组织化学分析:血管内皮生长因子(VEGF)、胰岛素样生长因子(IGF)、表皮生长因子(EGF)。根据创面持续时间将活检分为3组:1 ~≤90天组、2 ~ 91 ~ 365天组、3 ~ > 365天组。在持续时间不超过90天的伤口样本中,生长因子和受体的表达明显高于持续时间更长的伤口样本(p=0.0001)。生长因子及其受体的定量指标在创面持续91 ~ 365 d和超过365 d之间无显著差异。相关分析数据显示,VEGF、IGF、EGF及其受体的表达与创面时间呈显著负相关(p= 0.0001)。在持续时间小于90天的糖尿病足溃疡中,VEGF、IGF、EGF及其受体等生长因子的表达明显高于持续时间较长的伤口。揭示了生长因子的表达与溃疡持续时间的关系。所获得的数据可以解释慢性伤口结局的差异,以及某些情况下生长因子局部治疗效果的缺失。
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Growth factors and their receptors in neuropathic diabetic foot ulcers of different durations
BACKGROUND. Growth factors play a leading role in wound healing. Their deficiency in diabetic foot syndrome has been demonstrated in experimental studies, most of them performed on acute wounds. At the same time, currently the effectiveness of local therapy with growth factors of this category of patients, as well as indications and contraindications to it are contradictory and are a subject for discussion. There is no data on the content of growth factors in diabetic foot ulcers of various durations. Meanwhile, the results obtained will help to expand the understanding of the pathogenetic mechanisms of the course and outcomes of diabetic foot ulcers at different stages of healing.AIM: To evaluate the level of growth factors and their receptors in non-healing neuropathic diabetic foot ulcers of different duration.MATERIALS AND METHODS. 89 samples of neuropathic diabetic foot ulcers were examined. Immunohistochemical analysis of biopsies was performed using antibodies to the following growth factors and their receptors: vascular endothelial growth factor (VEGF), insulin-like growth factor (IGF), epidermal growth factor (EGF). Depending on the duration of the wound, the biopsies were divided into 3 groups: group 1 — ≤ 90 days, group 2 — 91-365 days, group 3 — > 365 days.RESULTS. In the samples of wounds, the duration of which did not exceed 90 days, the expression of growth factors and receptors to them was significantly higher than in wounds of longer duration (p=0.0001). There was no significant difference in quantitative indicators of the expression of growth factors and their receptors between wounds lasting from 91 to 365 days and more than 365 days. According to the data of correlation analysis, the expression of VEGF, IGF, EGF and their receptors has a significant negative dependence on the duration of the wound (p= 0.0001).CONCLUSION. In diabetic foot ulcers lasting less than 90 days, the expression of growth factors such as VEGF, IGF, EGF and their receptors is significantly higher than in wounds of longer duration. The dependence of the expression of growth factors on the duration of the ulcer was revealed. The data obtained may explain the differences in the outcomes of chronic wounds and the absence in some cases of the effect of local therapy with growth factors.
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来源期刊
Diabetes Mellitus
Diabetes Mellitus ENDOCRINOLOGY & METABOLISM-
CiteScore
1.90
自引率
40.00%
发文量
61
审稿时长
7 weeks
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