酿酒酵母聚半乳糖醛酸酶基因的克隆与表达

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI:10.1016/S0922-338X(98)80139-6

Naoto Hirose , Masao Kishida , Haruhiko Kawasaki , Takuo Sakai

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引用次数: 25

摘要

从酿酒酵母菌的突变体中克隆了一个内切多半乳糖醛酸酶基因，并鉴定了该基因的核苷酸序列。聚半乳糖醛酸酶基因全长1086 bp。当将聚半乳糖醛酸酶基因导入不能产生聚半乳糖醛酸酶的酿酒葡萄球菌菌株时，转化体分泌的聚半乳糖醛酸酶与产生聚半乳糖醛酸酶的突变体产生的酶相同。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Molecular cloning and expression of a polygalacturonase gene in Saccharomyces cerevisiae

An endo-polygalacturonase gene was cloned from a mutant of Saccharomyces cerevisiae that can produce a polygalacturonase, and the nucleotide sequence was identified. The polygalacturonase gene consists of 1086 bp. When the polygalacturonase gene was introduced into a strain of S. cerevisiae that cannot produce a polygalacturonase, the transformant secreted a polygalacturonase identical to that produced by the polygalacturonase-producing mutant.

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