通过pcPNA-S1核酸酶组合的序列特异性切割制备DNA/RNA杂交体

Nucleosides, Nucleotides and Nucleic Acids Pub Date : 2016-04-08 DOI:10.1080/15257770.2015.1131294

Kazuki Futai, J. Sumaoka, M. Komiyama

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引用次数: 1

摘要

通过将两条伪互补肽核酸(pcPNA)链与S1核酸酶结合，开发了一种位点选择性和双链切割DNA/RNA杂种的工具。杂交体中的DNA和RNA链都在所需的位点水解，以提供独特的粘性末端。通过T4 DNA连接酶将断裂片段直接与其他DNA/RNA杂交体连接，形成所需的重组DNA/RNA杂交体。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Fabrication of DNA/RNA Hybrids Through Sequence-Specific Scission of Both Strands by pcPNA-S1 Nuclease Combination

ABSTRACT By combining two strands of pseudo-complementary peptide nucleic acid (pcPNA) with S1 nuclease, a tool for site-selective and dual-strand scission of DNA/RNA hybrids has been developed. Both of the DNA and the RNA strands in the hybrids are hydrolyzed at desired sites to provide unique sticky ends. The scission fragments are directly ligated with other DNA/RNA hybrids by using T4 DNA ligase, resulting in the formation of desired recombinant DNA/RNA hybrids.

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Nucleosides, Nucleotides and Nucleic Acids

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