中药复方阿普沙对戊酸雌二醇诱导的多囊卵巢综合征Wistar白化大鼠模型的促排卵药理学评价

G. Begum, N. Anbu
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The research adhered to ethical principles and guidelines established by the committee responsible for overseeing and regulating animal experimentation, ensuring proper control and supervision. \nFemale Wistar Albino rats were selected as the preferred rodent species for the study. Polycystic ovary syndrome (PCOS) was induced in the animals by administering subcutaneous injection of 100μg Estardiol valerate (EV). The reproductive cycles of the rats were synchronized to ensure consistency. \nThe rats were divided into four groups, each containing six rats, as follows: \n \nGroup I: Normal Control animals received 1 ml/kg of Sodium Carboxymethyl Cellulose (CMC) solution. \nGroup II: Rats were orally administered Arputha Mathirai at a dosage of 100 mg/kg for 10 days. \nGroup III: Rats were orally administered Arputha Mathirai at a dosage of 200 mg/kg for 10 days. \nGroup IV: Received Clomiphene at a dosage of 10 mg/kg and served as the standard group. \n \nAt the end of the study, blood samples were collected from the rats through retro-orbital and cardiac puncture. The levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), estradiol, and progesterone were estimated using ELISA method. 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引用次数: 0

摘要

目的:本研究的目的是研究阿普塔·玛蒂莱(Arputha Mathirai),一种基于Siddha的草药制剂,对戊酸雌二醇(EV)诱导的Wistar白化大鼠多囊卵巢综合征(PCOS)的促排卵作用。研究地点:该研究在位于Thorapakkam, Chennai - 600092, Tamil Nadu的C.L. Baid Metha药学院进行。方法:Arputha Mathirai, Siddha草药制剂,按照良好生产规范(GMP)指南制备。在进行研究之前,获得了机构动物伦理委员会(IAEC)的批准。这项研究遵循了负责监督和管理动物实验的委员会制定的伦理原则和指导方针,确保了适当的控制和监督。选择雌性Wistar白化大鼠作为研究的首选啮齿类动物。以100μg戊酸雌二醇(EV)皮下注射诱导多囊卵巢综合征(PCOS)。大鼠的生殖周期同步以确保一致性。将大鼠分为4组,每组6只,按如下方法进行实验:1组:正常对照动物给予羧甲基纤维素钠(CMC)溶液1 ml/kg。II组:大鼠口服Arputha Mathirai,剂量为100mg /kg,持续10天。第三组:大鼠口服Arputha Mathirai,剂量为200mg /kg,持续10天。IV组:给予克罗米芬10 mg/kg剂量,作为标准组。在研究结束时,通过眶后穿刺和心脏穿刺采集大鼠血液样本。采用ELISA法测定黄体生成素(LH)、卵泡刺激素(FSH)、雌二醇、黄体酮水平。解剖实验大鼠卵巢进行组织病理学研究。结果:结果一致表明,与低剂量组相比,高剂量组(III组)在激素水平正常化方面的作用更为明显。然而,值得注意的是,低剂量组也表现出了有效性。虽然两组的结果与标准组(IV组)的结果并不接近,但药物Arputha Mathirai在实验组中显示出显着的潜力,支持其诱导排卵的功效。此外,组织病理学分析证实了该药的促排卵潜力,特别是高剂量组(III组)与低剂量组(II组)相比。总体而言,研究结果表明,Arputha Mathirai具有良好的促排卵活性,且高剂量效果更明显。结论:推测马提兰中的活性植物化合物可能在不同阶段发挥作用,恢复激素水平,逆转PCOS相关病理状况。这些天然化合物有调节激素水平和促进排卵的潜力。然而,重要的是要承认,关于这些植物化学物质解决这种情况的潜在原因的特定能力的公开证据不足。需要进一步研究这些化合物的作用机制及其在解决多囊卵巢综合征根本原因方面的潜力。
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Pharmacological Evaluation of Ovulation Inducing Potential of Siddha Herbomineral Formulation Arputha Mathirai on Estradiol Valerate Induced Poly Cystic Ovarian Syndrome Wistar Albino Rat Model
Aim: The aim of this study is to examine the potential of Arputha Mathirai, a tablet-based Siddha herbomineral formulation, in promoting ovulation in Wistar Albino rats with polycystic ovary syndrome (PCOS) induced by Estradiol Valerate (EV). Place of Study: The study took place at C.L. Baid Metha College of Pharmacy located in Thorapakkam, Chennai - 600 092, Tamil Nadu. Methodology: Arputha Mathirai, the Siddha herbomineral formulation, was prepared in accordance with Good Manufacturing Practices (GMP) guidelines. Prior to conducting the study, approval was obtained from the Institutional Animal Ethics Committee (IAEC). The research adhered to ethical principles and guidelines established by the committee responsible for overseeing and regulating animal experimentation, ensuring proper control and supervision. Female Wistar Albino rats were selected as the preferred rodent species for the study. Polycystic ovary syndrome (PCOS) was induced in the animals by administering subcutaneous injection of 100μg Estardiol valerate (EV). The reproductive cycles of the rats were synchronized to ensure consistency. The rats were divided into four groups, each containing six rats, as follows: Group I: Normal Control animals received 1 ml/kg of Sodium Carboxymethyl Cellulose (CMC) solution. Group II: Rats were orally administered Arputha Mathirai at a dosage of 100 mg/kg for 10 days. Group III: Rats were orally administered Arputha Mathirai at a dosage of 200 mg/kg for 10 days. Group IV: Received Clomiphene at a dosage of 10 mg/kg and served as the standard group. At the end of the study, blood samples were collected from the rats through retro-orbital and cardiac puncture. The levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), estradiol, and progesterone were estimated using ELISA method. Ovaries from the experimental rats were dissected out for histopathological studies. Results: The results consistently showed that the higher dosage of the drug (group III) had a more pronounced effect in normalizing hormone levels compared to the lower dosage group. However, it is important to note that the lower dosage group also exhibited effectiveness. While the results in both groups did not closely match those of the standard group (group IV), the drug Arputha Mathirai demonstrated significant potential in the experimental groups, supporting its efficacy in inducing ovulation. Furthermore, the histopathological analysis confirmed the drug's potential in inducing ovulation, particularly in the higher dosage group (group III) compared to the lower dosage group (group II). Overall, the findings suggest that Arputha Mathirai has promising ovulation-inducing activity, with the higher dosage showing more pronounced effects. Conclusion: It is hypothesized that the bioactive phytocompounds present in Arputha Mathirai may exert their effects at various stages to restore hormone levels and reverse the pathological condition associated with PCOS. These natural compounds have the potential to regulate hormone levels and promote ovulation. However, it is important to acknowledge that there is a scarcity of published evidence regarding the specific ability of these phytochemicals to address the underlying causes of the condition. Further research is needed to explore the mechanisms by which these compounds act and their potential in addressing the root causes of PCOS.
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