昆虫头角虫发育的生物化学研究。变态过程中14c -醋酸酯脂肪酸的体外生物合成

A.M Municio, J.M Odriozola, A Pinñeiro
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引用次数: 23

摘要

1.1. 头角虫卵、幼虫、蛹和成虫的匀浆中醋酸酯- 14c的掺入有明显差异。蛹期和幼虫发育阶段融合效果最好。NADPH以不同的方式影响乙酸的掺入。3.提高了幼虫匀浆的掺入率,降低了蛹匀浆的掺入率。柠檬酸降低了所有匀浆中乙酸的掺入。这种效应在蛹匀浆中更明显。4.4.柠檬酸盐对混合ATP-Mg2+在幼虫和蛹匀浆中活化作用的影响,分别增强和降低了幼虫和蛹匀浆的掺入量。在幼虫和蛹发育阶段脂肪酸合成调节的差异清楚地显示。
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Biochemistry of the development of the insect Ceratitis capitata. In vitro biosynthesis of fatty acids from 14C-acetate during metamorphosis

  • 1.

    1. Acetate-14C incorporation by the homogenates of the eggs, larvae, pupae and adults of Ceratitis capitata shows clear differences. The pupal and larvae stages of development exhibit the most effective incorporation.

  • 2.

    2. NADPH influences the acetate incorporation in a different way. It enhances the incorporation by larval homogenate whereas it produces a net decrease of the incorporation by pupa homogenate.

  • 3.

    3. Citrate decreases the acetate incorporation by all homogenates. This effect is higher in the pupal homogenate. As for the influence of citrate on the activatory effect of the mixture ATP-Mg2+ in larval and pupal homogenates, incorporation by larval and pupal homogenates is enhanced and decreased respectively.

  • 4.

    4. Differences in the regulation of fatty acid synthesis during larval and pupal stages of development are clearly shown.

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