利用产哭毒素内生细菌替代转基因植物保护马铃薯免受科罗拉多马铃薯甲虫侵害

Pub Date : 2022-03-01 DOI:10.17116/molgen20224001146

A. Sorokan, V. Alekseev

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引用次数: 0

摘要

摘要/ Abstract摘要:瘦肉毒霉(Leptinotarsa decemlineata, Ld)对化学和生物杀虫剂均产生抗药性。寻找能增加植物适应潜力的微生物共生体是很重要的。结果表明，苏云金芽孢杆菌B-5351的内生细胞能提高马铃薯Ld幼虫的死亡率，激活蛋白酶抑制剂及其蛋白产物基因的转录，而“低内生”的苏云金芽孢杆菌B-5659则能产生高效的虫毒素。

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Producing Cry-Toxins Endophytic Bacteria as an Alternative to Transgenic Plants to Protect Potato Plants against Colorado Potato Beetle

Abstract Leptinotarsa decemlineata ( Ld ) can develop resistance to chemical and biological insecticides. It is important to search for microbial symbionts of plants that increase their adaptive potential. It was shown that the endophytic cells of Bacillus thuringiensis B-5351 increases Ld larvae mortality and activates the transcription of genes of proteinase inhibitors and their protein products in potato plants after pest damage, in contrast to the “low endophytic” B. thuringiensis B-5659, which produces highly effective insectotoxins.

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