Ilaria Talli, Andrea Padoan, Stefania Moz, Filippo Navaglia, Mario Plebani, Daniela Basso
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引用次数: 0
摘要
背景：分子检测被认为是检测SARS-CoV-2的黄金标准。本研究旨在比较 P742H SARS-CoV-2 核酸多重检测试剂盒与意大利帕多瓦大学医院使用的 732HF 新型冠状病毒（2019-nCoV）核酸检测试剂盒和 TaqPath COVID-19 CEIVD RT-PCR 试剂盒在唾液样本中的性能：研究纳入了124份医护人员（HCW）于2022年10月至11月在意大利帕多瓦大学医院筛查项目中自行采集的唾液样本。使用病毒 DNA 和 RNA 提取试剂盒（Technogenetics，意大利洛迪）提取 RNA，并使用 P742H 和 732HF （Technogenetics，意大利洛迪）进行扩增。使用 MagNa Pure 96 DNA 和病毒 NA 小容量试剂盒（瑞士罗氏）提取 RNA，用于 TaqPath 分析（美国赛默飞世尔科技公司）。
Evaluation of a new molecular test for the detection of SARS-CoV-2 nucleic acid in salivary samples.
Background: Molecular testing is considered the gold standard for the detection of SARS-CoV-2. This study aimed to compare the performance of the P742H SARS-CoV-2 Nucleic Acid Multiplex Detection Kit in salivary samples, with respect to the 732HF Novel Coronavirus (2019-nCoV) Nucleic Acid Detection Kit and the TaqPath COVID-19 CEIVD RT-PCR Kit, used at University-Hospital of Padova, Italy.
Methods: One hundred twenty-four salivary samples selfcollected by healthcare workers (HCW) during the screening program at University-Hospital of Padova, Italy, from Oct to Nov 2022, were included in the study. RNA extraction was performed by Viral DNA and RNA Extraction Kit (Technogenetics, Lodi, Italy) and amplification by P742H and 732HF (Technogenetics, Lodi, Italy). RNA was extracted using MagNa Pure 96 DNA and Viral NA Small Volume Kit (Roche, Switzerland) for TaqPath analysis (Thermo Fisher Scientific, USA).
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