快速、经济、可扩展的唾液SARS-CoV-2检测

A. Hayden, Marcy L. Kuentzel, S. Chittur
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引用次数: 3

摘要

在这里,我们提出了一种廉价、快速、强大的基于RT-LAMP的SARS-CoV-2检测方法,该方法易于扩展,使护理点设施和临床实验室能够在30分钟内直接从患者唾液中确定结果,每个样本的成本不到2美元。该方法利用了广泛使用的试剂的新组合,这些试剂可以批量制备,电镀和冷冻,并且在收到样品之前保持稳定。这一创新大大缩短了准备时间,在使用384孔格式时,可在不到一小时内实现高通量自动化和高结果测试(包括设置)。通过使用双报告器(终点检测的酚红pH指示剂和实时的SYTO-9荧光染料),该分析还提供了结果的内部验证,并在仪器故障的情况下提供冗余。
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Rapid, Affordable and Scalable SARS-CoV-2 Detection from Saliva
Here we present an inexpensive, rapid, and robust RT-LAMP based SARS-CoV-2 detection method that is easily scalable, enabling point of care facilities and clinical labs to determine results from patients' saliva directly in 30 minutes for less than $2 a sample. The method utilizes a novel combination of widely available reagents that can be prepared in bulk, plated and frozen and remain stable until samples are received. This innovation dramatically reduces preparation time, enabling high-throughput automation and testing with time to results (including setup) in less than one hour for 96 patient samples simultaneously when using a 384 well format. By utilizing a dual-reporter (phenol red pH indicator for end-point detection and SYTO-9 fluorescent dye for real-time), the assay also provides internal validation of results and redundancy in the event of an instrument malfunction.
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