包埋芽孢杆菌sp. (Gen Bank MN 243657)与铜绿假单胞菌SU-1在家禽粪便降解中的比较评价与利用

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摘要

Bacillus sp. (Genbank MN243657)来自生物科学与纳米科学研究中心,P. aeruginosa SU-1用于本研究。首先,在RSM优化生物量之后,对生物量进行了孵育期、pH和温度的优化。获得的生物能够产生角化酶,发现它是64 KDa蛋白和56KDa蛋白。将优化条件下产生的生物质进行固定化,考察其降解角化酶的能力,并比较其效率。固定化铜绿假单胞菌SU-1对角蛋白和羽毛的降解效果优于芽孢杆菌(Genbank MN243657)。研究发现,它可以减少57.3%和41%的羽毛重量。结果表明,这两种固定化菌均可用于处理家禽废弃物,且P. aeruginosa SU-1的处理效率高于Bacillus sp. (Genbank MN243657)。
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A Comparative Evaluation and Utilization of Entrapped Bacillus sp. (Gen Bank MN 243657) and Pseudomonas aeruginosa SU-1 in Poultry Waste Degradation
Bacillus sp. (Genbank MN243657) was obtained from the Center for Bioscience and Nanoscience Research, and P. aeruginosa SU-1 were used in this study. Initially, biomass was optimized for the incubation period, pH, and temperature, following biomass optimization with RSM. The obtained organisms were able to produce keratinase, which was found to be a 64 KDa protein and 56KDa. Now the biomass produced using the optimized condition was immobilized and checked for its ability to degrade keratinase, and efficiency was compared. Immobilized P. aeruginosa SU-1 was degrading keratin and feather better than Bacillus sp. (Genbank MN243657). It was found to reduce featherweight by 57.3% and 41%. Hence it was found that both the immobilized bacterium can be used for poultry waste treatment, and P. aeruginosa SU-1 was more efficient than Bacillus sp. (Genbank MN243657).
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