{"title":"曼陀罗需要泛酸和腺嘌呤的营养缺陷体体细胞杂交的遗传互补。","authors":"P. Saxena, M. Mii, J. King","doi":"10.1139/G86-142","DOIUrl":null,"url":null,"abstract":"Auxotrophic complementation was achieved by protoplast fusion between two auxotrophic cell lines of Datura innoxia P. Mill. that required either pantothenate or adenine for their growth. Protoplast fusion was induced by polyethylene glycol enriched with calcium chloride at pH 6.8. Initial culture of fusion products in nonselective conditions increased the total number of somatic hybrids finally recovered under selective conditions. In addition to the prototrophy, the somatic hybrids regained the ability to turn green and some the ability to regenerate shoots on a cytokinin-supplemented medium. Both the parental cell lines grew as pale white callus without showing any sign of organogenesis, irrespective of the type and concentration of the cytokinin used. Approximately all the parental chromosomes (176 ± 8) were retained by the hybrid cell lines.Key words: protoplast, complementation, Datura.","PeriodicalId":9589,"journal":{"name":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","volume":"1 1","pages":"1022-1025"},"PeriodicalIF":0.0000,"publicationDate":"1986-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Genetic complementation by somatic hybridization between pantothenate- and adenine-requiring auxotrophs of Datura innoxia P. Mill.\",\"authors\":\"P. Saxena, M. Mii, J. King\",\"doi\":\"10.1139/G86-142\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Auxotrophic complementation was achieved by protoplast fusion between two auxotrophic cell lines of Datura innoxia P. Mill. that required either pantothenate or adenine for their growth. Protoplast fusion was induced by polyethylene glycol enriched with calcium chloride at pH 6.8. Initial culture of fusion products in nonselective conditions increased the total number of somatic hybrids finally recovered under selective conditions. In addition to the prototrophy, the somatic hybrids regained the ability to turn green and some the ability to regenerate shoots on a cytokinin-supplemented medium. Both the parental cell lines grew as pale white callus without showing any sign of organogenesis, irrespective of the type and concentration of the cytokinin used. Approximately all the parental chromosomes (176 ± 8) were retained by the hybrid cell lines.Key words: protoplast, complementation, Datura.\",\"PeriodicalId\":9589,\"journal\":{\"name\":\"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie\",\"volume\":\"1 1\",\"pages\":\"1022-1025\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1986-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1139/G86-142\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1139/G86-142","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
摘要
研究了曼陀罗(Datura innoxia P. Mill)两种营养缺失细胞系间原生质体融合实现营养缺失互补。它们的生长要么需要泛酸盐要么需要腺嘌呤。在pH为6.8的条件下,富氯化钙聚乙二醇诱导原生质体融合。融合产物在非选择条件下的初始培养增加了在选择条件下最终恢复的体细胞杂交种总数。除了原生营养外,体细胞杂交体在补充细胞分裂素的培养基上恢复了变绿的能力,一些还恢复了再生芽的能力。无论细胞分裂素的类型和浓度如何,两种亲本细胞系都生长成淡白色的愈伤组织,没有显示出任何器官发生的迹象。杂交细胞系几乎保留了所有亲本染色体(176±8条)。关键词:原生质体;互补;曼陀罗;
Genetic complementation by somatic hybridization between pantothenate- and adenine-requiring auxotrophs of Datura innoxia P. Mill.
Auxotrophic complementation was achieved by protoplast fusion between two auxotrophic cell lines of Datura innoxia P. Mill. that required either pantothenate or adenine for their growth. Protoplast fusion was induced by polyethylene glycol enriched with calcium chloride at pH 6.8. Initial culture of fusion products in nonselective conditions increased the total number of somatic hybrids finally recovered under selective conditions. In addition to the prototrophy, the somatic hybrids regained the ability to turn green and some the ability to regenerate shoots on a cytokinin-supplemented medium. Both the parental cell lines grew as pale white callus without showing any sign of organogenesis, irrespective of the type and concentration of the cytokinin used. Approximately all the parental chromosomes (176 ± 8) were retained by the hybrid cell lines.Key words: protoplast, complementation, Datura.