{"title":"用PCR法测定牛肉、绵羊和山羊肉的含量","authors":"Bursa Djafarli, Shalala K. Zeynalova","doi":"10.31073/onehealthjournal2023-i-03","DOIUrl":null,"url":null,"abstract":"\nIn Azerbaijan, all traditional dishes contain meat, which is considered a sign of a healthy life. The purpose of study was the identification of meat species and the presence of cow, sheep and goat meat in the composition of sausages. For this purpose, multiplex real-time PCR kits were used. The one of baselines of the study was to observe the cross reaction between sheep and goat meat, given their similarities. Also, ussualy sausage manufacturers write on labels that the composition includes cow and sheep meat, which is very important for the Muslim population. We used the PCR kit, based on four channels reading: FAM, JOE, ROX and CY5. The results have shown, though the manufactered company not include the presence of goat meat to the labels, the goat meat has been determined. The research confirmed, that the PCR method is the one of the sensitive techniques for determination of meat species' origin. \n","PeriodicalId":37300,"journal":{"name":"International Journal of One Health","volume":"92 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"DETERMINATION OF BEEF, SHEEP AND GOAT MEAT BY PCR\",\"authors\":\"Bursa Djafarli, Shalala K. Zeynalova\",\"doi\":\"10.31073/onehealthjournal2023-i-03\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\\nIn Azerbaijan, all traditional dishes contain meat, which is considered a sign of a healthy life. The purpose of study was the identification of meat species and the presence of cow, sheep and goat meat in the composition of sausages. For this purpose, multiplex real-time PCR kits were used. The one of baselines of the study was to observe the cross reaction between sheep and goat meat, given their similarities. Also, ussualy sausage manufacturers write on labels that the composition includes cow and sheep meat, which is very important for the Muslim population. We used the PCR kit, based on four channels reading: FAM, JOE, ROX and CY5. The results have shown, though the manufactered company not include the presence of goat meat to the labels, the goat meat has been determined. The research confirmed, that the PCR method is the one of the sensitive techniques for determination of meat species' origin. \\n\",\"PeriodicalId\":37300,\"journal\":{\"name\":\"International Journal of One Health\",\"volume\":\"92 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-01-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of One Health\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.31073/onehealthjournal2023-i-03\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Veterinary\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of One Health","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.31073/onehealthjournal2023-i-03","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Veterinary","Score":null,"Total":0}
In Azerbaijan, all traditional dishes contain meat, which is considered a sign of a healthy life. The purpose of study was the identification of meat species and the presence of cow, sheep and goat meat in the composition of sausages. For this purpose, multiplex real-time PCR kits were used. The one of baselines of the study was to observe the cross reaction between sheep and goat meat, given their similarities. Also, ussualy sausage manufacturers write on labels that the composition includes cow and sheep meat, which is very important for the Muslim population. We used the PCR kit, based on four channels reading: FAM, JOE, ROX and CY5. The results have shown, though the manufactered company not include the presence of goat meat to the labels, the goat meat has been determined. The research confirmed, that the PCR method is the one of the sensitive techniques for determination of meat species' origin.
期刊介绍:
International Journal of One Health publishes high quality and novelty papers focusing on One Health. Review articles are highly appreciated. All articles published by International Journal of One Health are made freely and permanently accessible online. All articles to International Journal of One Health are posted online immediately as they are ready for publication.