马尾藻藻酸盐聚寡糖(AOS)在抗致病性副溶血性弧菌、创伤弧菌和哈韦伊弧菌拮抗实验中的免疫刺激作用

IF 1.3 4区 化学 Q3 CHEMISTRY, MULTIDISCIPLINARY Main Group Chemistry Pub Date : 2022-01-24 DOI:10.3233/mgc-210116
E. Yudiati, N. Azhar, M. J. Achmad, S. Sunaryo, A. Susanto, B. Yulianto, R. Alghazeer, W. Alansari, G. Shamlan
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引用次数: 1

摘要

海藻酸盐是一种从马尾藻中提取的多糖,是一种有效的免疫刺激剂,具有抗菌活性,包括对弧菌的抗菌活性。弧菌属的细菌存在于淡水和海洋环境中,是水生动物和人类常见的感染性致病菌。在本研究中,我们测定了海藻酸钠多糖和寡糖(AOS)作为免疫刺激剂的能力,并对副溶血性弧菌、创伤弧菌和哈维弧菌进行了青蒿素激发试验和抗菌扩散盘试验。AOS由热加热产生。将干海藻酸钠从4.21 g称量到6.47 g,得率从21.05 g到32.35%不等。对褐藻酸盐多糖进行攻毒,有8个阳性结果。最高抑制区为12.962±3.623 mm。在18个试验中,AOS有12个阳性结果,最高抑制区为10.250±0.09 mm。包封的海藻酸盐对副溶血性弧菌、哈维弧菌、创伤弧菌的抑制作用,以及不添加弧菌的无攻毒试验的最佳抑制浓度分别为800 ppm(多糖)和600 ppm(低聚糖)。较低浓度的海藻酸寡糖效果较好,与海藻酸多糖相比,具有较好的抗菌和免疫促进作用。
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Alginate poly and oligosaccharide (AOS) from Sargassum sp. as immunostimulant in gnotobiotic artemia challenge tests and antibacterial diffusion disc assay against pathogenic Vibrio parahaemolyticus, V. vulnificus and V. harveyi
Alginate is a polysaccharide derived from Sargassum sp. and is a potent immunostimulant with antibacterial activity, including against Vibrio spp. This genus of bacteria is found in freshwater and marine environments and is a common infectious, pathogenic bacteria both for aquatic cultivans and humans. Here, we determined the ability of sodium alginate polysaccharides and oligosaccharides (AOS) to act as immunostimulants in Artemia challenge tests and antibacterial diffusion disc assays against Vibrio parahaemolyticus, V. vulnificus, and V. harveyi. The AOS was produced by thermal heating. Dry sodium alginates were weighed out from 4.21 to 6.47 grams with a yield varying from 21.05 to 32.35%. Alginate polysaccharides were challenged against V harveyi and showed 8 positive results. The highest inhibitor zone was 12.962±3.623 mm. Based on 18 tests, AOS showed 12 positive results, with the highest inhibitor zone being 10.250±0.09 mm. The encapsulated alginate against Vibrio parahaemolyticus, Vibrio harveyi, Vibrio vulnificus, and the non-challenged tests without any Vibrio spp. addition resulted in the best concentrations of 800 ppm (polysaccharide) and 600 ppm (oligosaccharide), respectively. The lower concentration of oligosaccharides alginate were more effective and has the potential to be superior as an antibacterial agent and immunestimulant, as opposed to alginate polysaccharide.
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来源期刊
Main Group Chemistry
Main Group Chemistry 化学-化学综合
CiteScore
2.00
自引率
26.70%
发文量
65
审稿时长
>12 weeks
期刊介绍: Main Group Chemistry is intended to be a primary resource for all chemistry, engineering, biological, and materials researchers in both academia and in industry with an interest in the elements from the groups 1, 2, 12–18, lanthanides and actinides. The journal is committed to maintaining a high standard for its publications. This will be ensured by a rigorous peer-review process with most articles being reviewed by at least one editorial board member. Additionally, all manuscripts will be proofread and corrected by a dedicated copy editor located at the University of Kentucky.
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