乔斯大学健康学生社区获得性耐甲氧西林金黄色葡萄球菌流行及药敏分析

P. O. Olorunfemi, J. Onaolapo, Y. Ibrahim
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引用次数: 1

摘要

金黄色葡萄球菌是一种剧毒病原体,目前不仅是世界范围内医院感染的最常见原因,而且越来越多地成为社区相关病原体。本研究旨在确定从尼日利亚乔斯市明显健康的大学生人群中分离出的耐甲氧西林金黄色葡萄球菌(MRSA)的流行率和敏感性。对217份尿液样本进行了金黄色葡萄球菌筛查。采用常规培养和生化方法(包括快速检测试剂盒(MicrogenID test kit))对分离物进行鉴定。使用临床和实验室标准研究所(CLSI)的方法对11种抗生素进行了敏感性分析。以肟西林和头孢西丁为标记物,对MRSA状态进行表型鉴定。收集的217份样品中,葡萄球菌阳性171份(78.8%),其中凝固酶阳性135份,属于金黄色葡萄球菌。其中73例经MicrogenID检测试剂盒鉴定为金黄色葡萄球菌。分离株对研究中使用的至少一种抗生素具有耐药性。73株金黄色葡萄球菌中71株对甲氧西林耐药。在社区分离株中观察到的易感模式表明,由这些菌株引起的任何感染暴发都可能对公共卫生构成威胁。
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Prevalence and antibiotic susceptibility of community acquired methicillin resistant Staphylococcus aureus from healthy students of University of Jos
Staphylococcus aureus is a virulent pathogen that is currently, not only the most common cause of nosocomial infections worldwide, but increasingly also, a community associated pathogen. This study aimed at determining the prevalence and susceptibility of methicillin resistant S. aureus (MRSA) isolated from apparently healthy university student population in Jos Nigeria. Two hundred and seventeen (217) urine samples were screened for Staphylococcus aureus. Isolates were characterized by conventional cultural and biochemical methods including rapid test kits (MicrogenID test kit). Their susceptibility profiles were determined against a panel of eleven antibiotics using the method of the Clinical and Laboratory Standard Institute (CLSI). The MRSA status were confirmed phenotypically using oxacillin and cefoxitin as markers. Of the 217 samples collected, 171 (78.8%) were positive for Staphylococcal species, while 135 of these were coagulase positive and were classed as S. aureus. Seventy-three (73) of these were identified as S. aureus with the MicrogenID test kit. Isolates were resistant to at least one of the antibiotics used in the study. Seventy-one (71) of 73 S. aureus isolates were methicillin resistant. The susceptibility pattern observed in community isolates implies that any outbreak of infections caused by these strains may pose a threat to public health.
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