{"title":"泡菜中乳杆菌的益生菌特性研究","authors":"Dong-Hun Chu, Moochang Kook","doi":"10.35732/CTLABP.2019.5.1.33","DOIUrl":null,"url":null,"abstract":"Lactobacillus pentosus A67 and L. planarum subsp. plantarum C2 was tested the probiotics characterization such as acid, bile acid and antibiotics resistance. Isolates were isolated from the traditional Korean food, collected from traditional markets and homes in Seoul and Gyeonggi Province, and were selected the isolates A67 and C2, which were the most antibacterial to E. coli. According to the analysis of 16S rRNA base sequences, the results showed that L. pentosus ATCC 8041 (similarity 100%), and L. plantarum subsp. plantarum ATCC 14917 (similarity 100%). L. pentosus A67 showed a survival rate of about 98.35% and the L. plantarum C2 showed a survival rate of about 95.62% at pH 2.5, respectively. L. pentosus A67 showed a survival rate of about 94.56% and the L. plantarum C2 showed a survival rate of about 88.81% on the 0.3% bile salt, respectively. L. pentosus A67 and L. plantarum C2 were survived by antibiotics such as the quinolone, aminoglycoside, macrolide, glycopeptide, tetracycline, cephalosporin and phenicillin families. On basis of these results, L. pentosus A67 and L. plantarum C2 are the candidates for useful probiotics.","PeriodicalId":10892,"journal":{"name":"Current Topic in Lactic Acid Bacteria and Probiotics","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Probiotics Characterization of Lactobacillus\\n Species Isolated from Kimchi\",\"authors\":\"Dong-Hun Chu, Moochang Kook\",\"doi\":\"10.35732/CTLABP.2019.5.1.33\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Lactobacillus pentosus A67 and L. planarum subsp. plantarum C2 was tested the probiotics characterization such as acid, bile acid and antibiotics resistance. Isolates were isolated from the traditional Korean food, collected from traditional markets and homes in Seoul and Gyeonggi Province, and were selected the isolates A67 and C2, which were the most antibacterial to E. coli. According to the analysis of 16S rRNA base sequences, the results showed that L. pentosus ATCC 8041 (similarity 100%), and L. plantarum subsp. plantarum ATCC 14917 (similarity 100%). L. pentosus A67 showed a survival rate of about 98.35% and the L. plantarum C2 showed a survival rate of about 95.62% at pH 2.5, respectively. L. pentosus A67 showed a survival rate of about 94.56% and the L. plantarum C2 showed a survival rate of about 88.81% on the 0.3% bile salt, respectively. L. pentosus A67 and L. plantarum C2 were survived by antibiotics such as the quinolone, aminoglycoside, macrolide, glycopeptide, tetracycline, cephalosporin and phenicillin families. On basis of these results, L. pentosus A67 and L. plantarum C2 are the candidates for useful probiotics.\",\"PeriodicalId\":10892,\"journal\":{\"name\":\"Current Topic in Lactic Acid Bacteria and Probiotics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Topic in Lactic Acid Bacteria and Probiotics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.35732/CTLABP.2019.5.1.33\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Topic in Lactic Acid Bacteria and Probiotics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.35732/CTLABP.2019.5.1.33","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
摘要
戊酸乳杆菌A67与扁平乳杆菌亚种。对植物C2进行了酸、胆汁酸及抗生素耐药性等益生菌鉴定。从首尔和京畿地区的传统市场和家庭采集的韩国传统食品中分离出菌株，筛选出对大肠杆菌抑菌力最强的菌株A67和C2。根据16S rRNA碱基序列分析，结果表明，L. pentosus ATCC 8041(相似度100%)与L. plantarum subsp;plantarum ATCC 14917(相似性100%)。在ph2.5条件下，L. pentosus A67和L. plantarum C2的存活率分别为98.35%和95.62%。L. pentosus A67和L. plantarum C2在0.3%胆汁盐上的存活率分别为94.56%和88.81%。L. pentosus A67和L. plantarum C2主要通过喹诺酮类、氨基糖苷类、大环内酯类、糖肽类、四环素类、头孢菌素类和青霉素类抗生素存活。基于这些结果，戊糖乳杆菌A67和植物乳杆菌C2是有益益生菌的候选菌。
Probiotics Characterization of Lactobacillus
Species Isolated from Kimchi
Lactobacillus pentosus A67 and L. planarum subsp. plantarum C2 was tested the probiotics characterization such as acid, bile acid and antibiotics resistance. Isolates were isolated from the traditional Korean food, collected from traditional markets and homes in Seoul and Gyeonggi Province, and were selected the isolates A67 and C2, which were the most antibacterial to E. coli. According to the analysis of 16S rRNA base sequences, the results showed that L. pentosus ATCC 8041 (similarity 100%), and L. plantarum subsp. plantarum ATCC 14917 (similarity 100%). L. pentosus A67 showed a survival rate of about 98.35% and the L. plantarum C2 showed a survival rate of about 95.62% at pH 2.5, respectively. L. pentosus A67 showed a survival rate of about 94.56% and the L. plantarum C2 showed a survival rate of about 88.81% on the 0.3% bile salt, respectively. L. pentosus A67 and L. plantarum C2 were survived by antibiotics such as the quinolone, aminoglycoside, macrolide, glycopeptide, tetracycline, cephalosporin and phenicillin families. On basis of these results, L. pentosus A67 and L. plantarum C2 are the candidates for useful probiotics.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
