植物提取物对细菌生长的影响及其可能的作用机制

M. Stanković, Jelena Z. Pribojac, Jelena N. Terzić, O. Stefanović
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引用次数: 0

摘要

薄荷(Mentha piperita)和茉莉(Melissa officinalis)都是著名的药用植物,在传统医学中有应用。本研究采用微量稀释法测定了胡椒分枝杆菌和officinalis分枝杆菌乙醇提取物对14株细菌的抑菌活性。两种植物乙醇提取物的最低抑菌浓度范围为0.312 ~ 20 mg/mL。标准菌株金黄色葡萄球菌ATCC 25923在浓度为0.312 mg/mL时和枯草芽孢杆菌ATCC 6633在浓度为1.25 mg/mL时对红毛杆菌乙醇提取物的敏感性最高。officinalis乙醇提取物在浓度为0.625 mg/mL时对标准菌株金黄色葡萄球菌ATCC 25923和枯草芽孢杆菌ATCC 6633具有抑菌活性。除上述标准菌株外,该菌株对食品中变形杆菌分离物的活性为0.312 mg/mL,对伤口中变形杆菌分离物的活性为0.625 mg/mL。考察了马蹄草乙醇提取物对细菌细胞膜通透性的作用机制。通过蛋白质的释放量和结晶紫结合的百分比来测定提取物对细胞膜通透性增加的影响。officinalis的乙醇提取物已被证明在细胞膜水平上对以下铜绿假单胞菌、金黄色葡萄球菌和肠球菌菌株起作用。
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EFFECT OF PLANT EXTRACTS ON BACTERIAL GROWTH AND POTENTIAL MECHANISM OF ACTION
Mentha piperita and Melissa officinalis are both well-known medicinal plants that have applications in traditional medicine. In this research the antibacterial activity of the ethanol extracts of M. piperita and M. officinalis was examined against 14 bacterial strains via the microdilution method. Minimum inhibitory concentrations of ethanol extracts of both plant species ranged from 0.312 to 20 mg/mL. Standard strains of Staphylococcus aureus ATCC 25923 at a concentration of 0.312 mg/mL and Bacillus subtilis ATCC 6633 at a concentration of 1.25 mg/mL showed the highest sensitivity to the ethanol extract of M. piperita. Ethanol extract of M. officinalis showed antibacterial activity on standard strains of S. aureus ATCC 25923 and B. subtilis ATCC 6633 at a concentration of 0.625 mg/mL. In addition to the mentioned standard strains, it showed activity on the isolate from the food Proteus spp. at a concentration of 0.312 mg/mL and isolate from the wound Proteus mirabilis at a concentration of 0.625 mg/mL. Mechanism of action of the ethanol extract of M. officinalis was examined on the permeability of the bacterial cell membrane. The effect of the extract on the increased permeability of the cell membrane was measured based on the release of proteins and the percentage of crystal violet binding. Ethanol extract of M. officinalis has been shown to act at the level of the cell membrane in the following bacterial strains of Pseudomonas aeruginosa, S. aureus and Enterococcus spp.
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